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Dysregulation of cholesterol homeostasis in human prostate cancer through loss of ABCA1

ABSTRACT: We conducted gene expression profilling of common prostate cell lines for comparison with genome-wide DNA methylation profiles for the same cell lines to study the impact of differential DNA methylation in prostate cancer. Total RNA was extracted from 3 biological replicate sets of PrEC, RWPE-1, DU 145, and LNCaP cells. This Series represents the gene expression profiling data only. The methyl-Seq data were submitted directly to SRA and are represented by accession SRA049689.1.

ORGANISM(S): Homo sapiens

SUBMITTER: Angela Ting

PROVIDER: E-GEOD-35401 | biostudies-arrayexpress |

REPOSITORIES: biostudies-arrayexpress

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Dysregulation of cholesterol homeostasis in human prostate cancer through loss of ABCA1.

Lee Byron H BH Taylor Margaret G MG Robinet Peggy P Smith Jonathan D JD Schweitzer Jessica J Sehayek Ephraim E Falzarano Sara M SM Magi-Galluzzi Cristina C Klein Eric A EA Ting Angela H AH

Cancer research 20121211 3

Recent epidemiologic data show that low serum cholesterol level as well as statin use is associated with a decreased risk of developing aggressive or advanced prostate cancer, suggesting a role for cholesterol in aggressive prostate cancer development. Intracellular cholesterol promotes prostate cancer progression as a substrate for de novo androgen synthesis and through regulation of AKT signaling. By conducting next-generation sequencing-based DNA methylome analysis, we have discovered marked ...[more]

PMID: 23233737

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Dysregulation of cholesterol homeostasis in human prostate cancer through loss of ABCA1

Project description:We conducted gene expression profilling of common prostate cell lines for comparison with genome-wide DNA methylation profiles for the same cell lines to study the impact of differential DNA methylation in prostate cancer.

2012-12-19 | GSE35401 | GEO

Patterns of aberrant DNA methylation after toxicant-induced malignant transformation (MeDIP-chip dataset 1)

Project description:Aberrant DNA methylation is frequently observed in cancer. The aim of this study was to determine how DNA methylation is changed after toxicant-induced malignant transformation. This study also puts the DNA methylation changes into context with respect to the aberrant DNA methylation events that occur in bladder and prostate carcinogenesis not associated with toxicant exposure. Immortalized UROtsa (n=3) and RWPE-1 (n=2) are compared to normal HUC (n=2) and PrEC (n=2), respectively. Arsenite (n=1), monomethylarsonous acid (n=2) or cadmium (n=1) transformed UROtsa are compared to parental UROtsa (n=3). Arsenite (n=2), cadmium (n=1) or MNU (n=1) transformed RWPE-1 cells are compared to parental RWPE-1 cells (n=2). Clinical bladder tumor biopsies (n=6), urothelial carcinoma cell lines (n=2) and prostate cancer cell lines (n=3) are compared to thier normal tissue counterparts HUC (n=2) and PrEC (n=2). Immunoprecipitation using anti-methylcytosine (5MeC) antibody.

2012-06-25 | E-GEOD-38926 | biostudies-arrayexpress

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2012-06-25 | E-GEOD-38928 | biostudies-arrayexpress

miRNA expression in LNCaP, PC3, Du-145 and RWPE-1 cell lines

Project description:MiRNAs are small non-coding RNAs that regulate the expression of specific mRNA targets mainly by translational repression, mRNA deadenylation or cleavage. This series is meant to identify miRNAs deregulated in prostate cancer (PCa) by comparing the PCa cell lines LNCaP, PC3 and Du-145 to the normal prostate epithelial cell line RWPE-1. We analyzed three arrays each for LNCaP, PC3, Du-145 and RWPE-1 cell lines

2012-03-08 | E-GEOD-17317 | biostudies-arrayexpress

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2012-06-25 | E-GEOD-38929 | biostudies-arrayexpress

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Characterisation and reproducibility of theHumanMethylationEPIC v2.0 BeadChip for DNAmethylation profiling [EPIC v2]

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