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Genome-wide analysis of human macrophages stimulated with IL-4 (20ng/ml) (Illumina)

ABSTRACT: Analysis of alternative activation of macrophages at gene expression level. The study forms part of a wider study where we compare the effects of IL-4 in different human and mouse macrophages. Our results support the notion that in vitro culture conditions greatly affect the macrophage response to IL-4. Total RNA obtained from human monocyte derived macrophages upon exposure to 20 ng/ml of IL-4 for 24 hours. Monocytes were induced to mature into macrophages using X Vivo 10 medium supplemented with 1% autologous serum.

ORGANISM(S): Homo sapiens

SUBMITTER: Fernando Martinez Estrada

PROVIDER: E-GEOD-35433 | biostudies-arrayexpress |

REPOSITORIES: biostudies-arrayexpress

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Genome-wide analysis of human macrophages stimulated with IL-4 (10ng/ml) (Illumina)

Project description:Analysis of alternative activation of macrophages at gene expression level. The study forms part of a wider study where we compare the effects of IL-4 in different human and mouse macrophages. Our results support the notion that in vitro culture conditions greatly affect the macrophage response to IL-4. Total RNA obtained from human monocyte derived macrophages upon exposure to 10 ng/ml of IL-4 for 48 hours. Monocytes were induced to mature into macrophages using X Vivo 10 medium supplemented with 1% autologous serum.

2012-09-29 | E-GEOD-35434 | biostudies-arrayexpress

Genome-wide analysis of human macrophages stimulated with IL-4

Project description:This SuperSeries is composed of the following subset Series: GSE5099: Expression Data from Macrophage Maturation and Polarization Experiment GSE35433: Genome-wide analysis of human macrophages stimulated with IL-4 (20ng/ml) (Illumina) GSE35434: Genome-wide analysis of human macrophages stimulated with IL-4 (10ng/ml) (Illumina) GSE35435: Genome-wide analysis of mouse macrophages stimulated with IL-4 (bone marrow macrophages) (Affymetrix) GSE35436: Genome-wide analysis of mouse macrophages stimulated with IL-4 (Biogel and thioglycollate macrophages) (Affymetrix) Refer to individual Series

2012-09-29 | E-GEOD-35495 | biostudies-arrayexpress

Genome-wide analysis of human macrophages stimulated with IL-4 (10ng/ml) (Illumina)

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Genome-wide analysis of human macrophages stimulated with IL-4 (20ng/ml) (Illumina)

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RNAseq analysis of γδ T-APCs generated in the presence of IL-2, IL-15 or IL-21, aiming to define the molecular mechanism underlying selective induction of IL-10 in naïve and memory CD4+ T-cells

Project description:This experiment involved isolating monocytes and irradiating them before co-culturing with autologous γδ T-cells at a 1:10 ratio. The cell culture experiments were analysed after HMB-PP activation only, or in combination with different interleukins (IL-2, IL-15, or IL-21). After three days, CD3+ Vδ2+ T-cells were selected via FACs (> 99% purity).

2024-04-01 | E-MTAB-13875 | biostudies-arrayexpress

Genome-wide analysis of mouse macrophages stimulated with IL-4 (Biogel and thioglycollate macrophages) (Affymetrix)

Project description:Analysis of alternative activation of macrophages at gene expression level. The study forms part of a wider study where we compare the effects of IL-4 in different human and mouse macrophages. Our results support the notion that in vitro culture conditions greatly affect the macrophage response to IL-4. Total RNA obtained from Thioglycollate and Biogel elicited peritoneal macrophages exposed to 20 ng/ml of IL-4 for 18 hours. Biogel and thioglycollate elicited macrophages were stimulated with the Th2 cytokine IL-4, for RNA extraction and hybridization on Affymetrix microarrays.

2012-09-29 | E-GEOD-35436 | biostudies-arrayexpress

Human monocyte-derived dendritic cells: Control vs Tolerogenic

Project description:Transcriptional profiling of human dendritic cells (DC) comparing control (DC generated with GM-CSF plus IL-4) with three different treatments of tolerogenic (DC generated with GM-CSF plus IL-4 and IL-10, or IL-4, IL-10, and IL-6, or IL-4, IL-10, and TGF-b1) Three two-condition experiments, control (N) vs tolerogenic DC with three different treatments. Pool of 4 indivuduals for each condition. One replicate per array.

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transcriptome of M1 and M2 stimuli

Project description:We compare M1 and M2 stimuli in human macrophages. Total RNA obtained from autologous serum monocyte-derived macrophages exposed to key cytokines, Lps, Glucocorticoid receptor ligand or left untreated.

2014-01-03 | E-GEOD-51446 | biostudies-arrayexpress

Genome-wide analysis of mouse macrophages stimulated with IL-4 (bone marrow macrophages) (Affymetrix)

Project description:Analysis of alternative activation of macrophages at gene expression level. The study forms part of a wider study where we compare the effects of IL-4 in different human and mouse macrophages. Our results support the notion that in vitro culture conditions greatly affect the macrophage response to IL-4. Total RNA obtained from bone marrow derived macrophages upon exposure to 20 ng/ml of IL-4 for 18 hours. Bone marrow derived macrophages were stimulated with the Th2 cytokine IL-4, for RNA extraction and hybridization on Affymetrix microarrays.

2012-09-29 | E-GEOD-35435 | biostudies-arrayexpress

Genome-wide analysis of mouse macrophages stimulated with IL-4 (Biogel and thioglycollate macrophages) (Affymetrix)

Project description:Analysis of alternative activation of macrophages at gene expression level. The study forms part of a wider study where we compare the effects of IL-4 in different human and mouse macrophages. Our results support the notion that in vitro culture conditions greatly affect the macrophage response to IL-4. Total RNA obtained from Thioglycollate and Biogel elicited peritoneal macrophages exposed to 20 ng/ml of IL-4 for 18 hours.

2012-09-29 | GSE35436 | GEO

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