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Yarrowia lipolytica biolipid production during D-stat setup. [Part 2]

ABSTRACT: Second part of the time-course transcriptomic profilling of the oleaginous yeast Yarrowia lipolytica, obtained during a controlled decellerostat (D-stat) setup. A nitrogen limitation was applied during the course of the D-stat to initiate and control de novo biolipid synthesis. Yarrowia lipolytica Agilent microarray, one-color staining. Each time sample was spotted in triplicate (except for sample 26, for which a technical replicate of replicate r1 was performed).

ORGANISM(S): Yarrowia lipolytica

SUBMITTER: Nicolas Morin

PROVIDER: E-GEOD-35446 | biostudies-arrayexpress |

REPOSITORIES: biostudies-arrayexpress

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Yarrowia lipolytica biolipid production during D-stat setup. [Part 1]

Project description:First part of the time-course transcriptomic profilling of the oleaginous yeast Yarrowia lipolytica, obtained during a controlled decellerostat (D-stat) setup. A nitrogen limitation was applied during the course of the D-stat to initiate and control de novo biolipid synthesis. Yarrowia lipolytica Agilent microarray, one-color staining. Each time sample was spotted in triplicate. A reference condition was spotted in quadruplicate, based on a RNA pool from three samples obtained during the biomass production phase.

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Yarrowia lipolytica biolipid production during D-stat setup.

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Dynamics of two oscillation phenotypes in S. cerevisiae reveal a network of genome-wide transcriptional and cell cycle oscillators.

Project description:This SuperSeries is composed of the following subset Series: GSE30051: Dynamics of two oscillation phenotypes in S. cerevisiae[2hr] GSE30052: Dynamics of two oscillation phenotypes in S. cerevisiae[4hr]. Refer to individual Series

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Project description:Whole transcriptome assessment of the Yersinia pseudotuberculosis strain YPIII. The Y. pseudotuberculosis csrA regulon was determined in Yersinia minimal minimum developed for the study. CsrA is a key regulator coordinating virulence and metabolism. Y. pseudotuberculosis YPIII or the isogenic csrA mutant strain were cultivated at 25M-BM-0C under aeration on a rotary shaker. First pre-cultures were grown in a 1:1 mixture of HAMM-bM-^@M-^Ys F-12 Nutrient Mixture (Invitrogen, Carlsbad, US) and liquid DMEM medium (Biochrom, Berlin, DE). Second pre-cultures and main cultures were grown in a Yersinia minimal medium (YMM). The analysis comprised three biological replicates for each strain. In addition, samples, taken at three different time points of the exponential growth phase, were used to validate constant expression during the cultivation. Total RNA was extracted using SV Total RNA Isolation System (Promega). The samples were treated with RNase-free DNase (Roche Applied Science) and the quality of the RNA was confirmed by the lack of PCR amplification of the hns gene and by using an Agilent 2100 Bioanalyzer.

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RNA-seq of the prototrophic non-hyphal smooth-33 mutant (FKP514) and its prototrophic control strain (FKP391)

Project description:FKP503 is a non-hyphal mutant strain (smooth-33) that was obtained from multiple passages of its parental strain FKP355. FKP355 and FKP503 are leucine auxotrophic strains: for further investigation prototropic strains FKP391 and FKP514 were constructed. To compare transcriptomic changes, FKP391 and FKP514 were cultured in chemostats under conditions stimulating lipid accumulation (nitrogen restriction).

2018-11-26 | E-MTAB-7400 | biostudies-arrayexpress

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