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The characteristics of genome-wide DNA methylation in naM-CM-/ve CD4+ T cells of patients with psoriasis and atopic dermatitis

ABSTRACT: Psoriasis and atopic dermatitis (AD) are characterized by polarized CD4+ T cell responses. During the polarization of naM-CM-/ve CD4+ T cells, DNA methylation plays an important role in the regulation of gene transcription. In this study, we profiled the genome-wide DNA methylation status of naM-CM-/ve CD4+ T cells in patients with psoriasis or AD and healthy controls using a ChIP-seq method. As a result, twenty-six regions in the genome ranging in size from 10 to 70 kb were markedly hypomethylated in patients with psoriasis. These regions were mostly pericentromeric on 10 different chromosomes and overlapped with various strong epigenomic signals, such as histone modifications and transcription factor binding sites, that were observed in the ENCODE project. Gene-centric analysis indicated that the promoter regions of 124 genes on the X chromosome had dramatically elevated methylation levels in patients with psoriasis as compared to those from healthy controls (> 4-fold). Moreover, immune-related genes on the X chromosome had higher hypermethylation than other genes (P < 0.05). These findings imply that methylation changes in naM-CM-/ve CD4+ T cells may affect CD4+ T cell polarization, especially in the pathogenesis of psoriasis. Keywords: Psoriasis, Atopic dermatitis, DNA methylation, naM-CM-/ve CD4+ T cells Sample submission examines DNA methylation from human naive CD4+ T cells in patients with psoriasis and atopic dermatitis. Note: Raw data available only for Sample GSM871288.

ORGANISM(S): Homo sapiens

SUBMITTER: Sin-Gi Park

PROVIDER: E-GEOD-35582 | biostudies-arrayexpress |

REPOSITORIES: biostudies-arrayexpress

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The characteristics of genome-wide DNA methylation in naïve CD4+ T cells of patients with psoriasis or atopic dermatitis.

Han Jihye J Park Sin-Gi SG Bae Jae-Bum JB Choi JungKyoon J Lyu Jae-Myun JM Park Sung Hee SH Kim Hei Sung HS Kim Young-Joon YJ Kim Sangsoo S Kim Tae-Yoon TY

Biochemical and biophysical research communications 20120430 1

Psoriasis and atopic dermatitis (AD) are skin diseases that are characterized by polarized CD4+ T cell responses. During the polarization of naïve CD4+ T cells, DNA methylation plays an important role in the regulation of gene transcription. In this study, we profiled the genome-wide DNA methylation status of naïve CD4+ T cells in patients with psoriasis or AD and healthy controls using a ChIP-seq method. Only psoriasis patient T cells, not those of AD patients, showed distinct hypomethylation ( ...[more]

PMID: 22564738

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Dataset Information

The characteristics of genome-wide DNA methylation in naM-CM-/ve CD4+ T cells of patients with psoriasis and atopic dermatitis

Publications

The characteristics of genome-wide DNA methylation in naïve CD4+ T cells of patients with psoriasis or atopic dermatitis.

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