Ontology highlight
ABSTRACT:
ORGANISM(S): Lactobacillus plantarum
SUBMITTER: Michiel Wels
PROVIDER: E-GEOD-35754 | biostudies-arrayexpress |
REPOSITORIES: biostudies-arrayexpress
Leimena Milkha M MM Wels Michiel M Bongers Roger S RS Smid Eddy J EJ Zoetendal Erwin G EG Kleerebezem Michiel M
Applied and environmental microbiology 20120406 12
RNA sequencing is starting to compete with the use of DNA microarrays for transcription analysis in eukaryotes as well as in prokaryotes. The application of RNA sequencing in prokaryotes requires additional steps in the RNA preparation procedure to increase the relative abundance of mRNA and cannot employ the poly(T)-primed approach in cDNA synthesis. In this study, we aimed to validate the use of RNA sequencing (direct cDNA sequencing and 3'-untranslated region [UTR] sequencing) using Lactobaci ...[more]