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Affymetrix SNP array data for a melanoma lesion and a cellline derived thereof

ABSTRACT: Here we report on a bulk culture termed MCM1, which was derived from a 61 year old male diagnosed with a nodular melanoma stage T2a, N0. At passage three of culturing, DNA was isolated and Affymetrix SNP6.0 array hybridizations were performed. Cultured MCM1 cells revealed overall identical copy number gains and losses as compared with the primary patient-derived lesion and were therefore used in this study. Affymetrix SNP arrays were performed according to the manufacturer's directions on DNA, which was extracted from a patient derived melanoma sample and from a cellline generated from the same melanoma sample at passage three.

ORGANISM(S): Homo sapiens

SUBMITTER: Mario Mikula

PROVIDER: E-GEOD-35991 | biostudies-arrayexpress |

REPOSITORIES: biostudies-arrayexpress

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MET expression in melanoma correlates with a lymphangiogenic phenotype.

Swoboda Alexander A Schanab Oliver O Tauber Stefanie S Bilban Martin M Berger Walter W Petzelbauer Peter P Mikula Mario M

Human molecular genetics 20120507 15

Melanomas contain high frequencies of tumorigenic cells and their tumorigenic capacity resides in several distinct subpopulations within melanoma. Since their metastatic potential is linked to their ability to recruit lymphatic vessels, we aimed at identifying lymphangiogenic subpopulations by comparative in vitro analysis of single cell clones derived from a melanoma of a single patient. Selected lymphangiogenic clones were then grafted into severe combined immunodeficient mice, where they indu ...[more]

PMID: 22570180

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