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Transcription profiling of mouse cochlear hair cell line

ABSTRACT: This study explores the profiles of genes expressed during conditional differentiation of inner ear hair cell precursors in vitro. The cell line UB/OC-1 was derived from mouse embryonic organs of Corti (Rivolta et al, Proc. R. Soc. Lond. B 265: 1595-1603, 1998) and it can be induced to differentiate upon a temperature shift. The study has been replicated in two separate populations of cells, named Exp A and Exp B. Exp A includes daily time points from 0 to 9 days, then 11 and 14 days. Exp B includes days 0, 2, 4, and 9. The manuscript associated to this work is Rivolta et al., Genome Research 12(7):1091-1099, 2002.

ORGANISM(S): Mus musculus

SUBMITTER: Marcelo Nicolas Rivolta

PROVIDER: E-GEOD-36 | biostudies-arrayexpress |

REPOSITORIES: biostudies-arrayexpress

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Transcript profiling of functionally related groups of genes during conditional differentiation of a mammalian cochlear hair cell line.

Rivolta Marcelo N MN Halsall Antony A Johnson Claire M CM Tones Michael A MA Holley Matthew C MC

Genome research 20020701 7

We have used Affymetrix high-density gene arrays to generate a temporal profile of gene expression during differentiation of UB/OC-1, a conditionally immortal cell line derived from the mouse cochlea. Gene expression was assessed daily for 14 days under differentiating conditions. The experiment was replicated in two separate populations of cells. Profiles for selected genes were correlated with those obtained by RT-PCR, TaqMan analysis, immunoblotting, and immunofluorescence. The results sugges ...[more]

PMID: 12097346

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UB/OC-1 differentiation

Project description:This study explores the profiles of genes expressed during conditional differentiation of inner ear hair cell precursors in vitro. The cell line UB/OC-1 was derived from mouse embryonic organs of Corti (Rivolta et al, Proc. R. Soc. Lond. B 265: 1595-1603, 1998) and it can be induced to differentiate upon a temperature shift. The study has been replicated in two separate populations of cells, named Exp A and Exp B. Exp A includes daily time points from 0 to 9 days, then 11 and 14 days. Exp B includes days 0, 2, 4, and 9. The manuscript associated to this work is Rivolta et al., Genome Research 12(7):1091-1099, 2002. Keywords: time-course

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