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Lung Natural Helper cells

ABSTRACT: Natural Helper cells constitute a unique lineage of Th2-cytokine producting innate lymphocytes, here we characterize the gene expression profile of non-stimulated or PMA/ionomycin-stimulated Natural Helper cells from naive C57Bl/6 mouse lungs. We used microarrays to detail the gene expression profile of stimulated and unstimulated lung Natural Helper cells in mice. Lineage(-)Sca-1(+)c-Kit(-/low)CD127(+)CD25(+) Lung Natural Helper cells were purified from naive 6-8 week old B6 mice by FACS. RNA was extracted immediately from un-stimulated Natural Helper cells after FACS. Stimulated Natural Helper cells were cultured with PMA/ionomycin for 3 days followed by RNA extraction.

ORGANISM(S): Mus musculus

SUBMITTER: Timotheus Halim

PROVIDER: E-GEOD-36057 | biostudies-arrayexpress |

REPOSITORIES: biostudies-arrayexpress

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Lung Natural Helper cells

Project description:Natural Helper cells constitute a unique lineage of Th2-cytokine producting innate lymphocytes, here we characterize the gene expression profile of non-stimulated or PMA/ionomycin-stimulated Natural Helper cells from naive C57Bl/6 mouse lungs. We used microarrays to detail the gene expression profile of stimulated and unstimulated lung Natural Helper cells in mice.

2012-02-24 | GSE36057 | GEO

RNA-seq of mouse T cells exposed to Schistosoma mansoni with transwell barrier.

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FOXP3 binding sites in promoter regions of human Jurkat T cells

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