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Extrachromosomal microDNAs and chromosomal microdeletions in normal tissues

ABSTRACT: We have identified tens of thousands of short extrachromosomal circular DNAs (microDNA) in mouse tissues as well as mouse and human cell lines. These microDNAs are 200-400 bp long, derived from unique non-repetitive sequence and are enriched in the 5' untranslated regions of genes, exons and CpG islands. Chromosomal loci that are enriched sources of microDNA in adult brain are somatically mosaic for micro-deletions that appear to arise from the excision of microDNAs. Germline microdeletions identified by the "Thousand Genomes" project may also arise from the excision of microDNAs in the germline lineage. We have thus identified a new DNA entity in mammalian cells and provide evidence that their generation leaves behind deletions in different genomic loci. Circular DNA profiling by high throughput sequencing

ORGANISM(S): Homo sapiens

SUBMITTER: anindya dutta

PROVIDER: E-GEOD-36088 | biostudies-arrayexpress |

REPOSITORIES: biostudies-arrayexpress

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Extrachromosomal microDNAs and chromosomal microdeletions in normal tissues.

Shibata Yoshiyuki Y Kumar Pankaj P Layer Ryan R Willcox Smaranda S Gagan Jeffrey R JR Griffith Jack D JD Dutta Anindya A

Science (New York, N.Y.) 20120308 6077

We have identified tens of thousands of short extrachromosomal circular DNAs (microDNA) in mouse tissues as well as mouse and human cell lines. These microDNAs are 200 to 400 base pairs long, are derived from unique nonrepetitive sequence, and are enriched in the 5'-untranslated regions of genes, exons, and CpG islands. Chromosomal loci that are enriched sources of microDNA in the adult brain are somatically mosaic for microdeletions that appear to arise from the excision of microDNAs. Germline ...[more]

PMID: 22403181

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