Unknown,Transcriptomics,Genomics,Proteomics

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Comparison of the gene expression profiles of a recombinant protein producing Hek 293 cell line and its non-producing parental cell line.

ABSTRACT: Comparison of the gene expression profiles of a recombinant protein producing Hek 293 cell line (referred to as producer) and its non-producing parental cell line Hek293F (referred to as non-producer). The parental cell line was obtained from Invitrogen, Carlsbad, CA. The producer was transfected with a heavy chain variable region fused to the Fc region of a human IgG (dAb-Fc). The aim of this study was to gain a better understanding of the process of recombinant protein production in Hek293 cells and to identify targets for the engineering of an improved host cell line. Duplicate samples for RNA extraction were taken at four time points (approximately 30, 43, 51 and 67 hours after inoculation of the bioreactor) during the exponential phase of batch bioreactor cultures. Only one of these technical duplicates was analysed. Three bioreactor cultures were performed for each cell line representing three biological replicates resulting in 12 (3 x 4) samples per cell line.

ORGANISM(S): Homo sapiens

SUBMITTER: Stefanie Dietmair

PROVIDER: E-GEOD-36094 | biostudies-arrayexpress |

REPOSITORIES: biostudies-arrayexpress

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Publications

A multi-omics analysis of recombinant protein production in Hek293 cells.

Dietmair Stefanie S Hodson Mark P MP Quek Lake-Ee LE Timmins Nicholas E NE Gray Peter P Nielsen Lars K LK

PloS one 20120824 8

Hek293 cells are the predominant hosts for transient expression of recombinant proteins and are used for stable expression of proteins where post-translational modifications performed by CHO cells are inadequate. Nevertheless, there is little information available on the key cellular features underpinning recombinant protein production in Hek293 cells. To improve our understanding of recombinant protein production in Hek293 cells and identify targets for the engineering of an improved host cell ...[more]

PMID: 22937046

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Dataset Information

Comparison of the gene expression profiles of a recombinant protein producing Hek 293 cell line and its non-producing parental cell line.

Publications

A multi-omics analysis of recombinant protein production in Hek293 cells.

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