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Expression profiles of primary cortical neurons after knocking down Fus

ABSTRACT: To clarify the functional properties of FUS, we established the differentially expressed alternative exons in FUS-silenced primary cortical neurons by using exon-sensitive microarray technology. We analyzed total RNA of primary cortical neuron infected with lentivirus expressing shRNA against mouse Fus or control. RNA was harvested 11 days after transfection.

ORGANISM(S): Mus musculus

SUBMITTER: Akio Masuda

PROVIDER: E-GEOD-36153 | biostudies-arrayexpress |

REPOSITORIES: biostudies-arrayexpress

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Position-dependent FUS-RNA interactions regulate alternative splicing events and transcriptions.

Ishigaki Shinsuke S Masuda Akio A Fujioka Yusuke Y Iguchi Yohei Y Katsuno Masahisa M Shibata Akihide A Urano Fumihiko F Sobue Gen G Ohno Kinji K

Scientific reports 20120724

FUS is an RNA-binding protein that regulates transcription, alternative splicing, and mRNA transport. Aberrations of FUS are causally associated with familial and sporadic ALS/FTLD. We analyzed FUS-mediated transcriptions and alternative splicing events in mouse primary cortical neurons using exon arrays. We also characterized FUS-binding RNA sites in the mouse cerebrum with HITS-CLIP. We found that FUS-binding sites tend to form stable secondary structures. Analysis of position-dependence of FU ...[more]

PMID: 22829983

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