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Expression data from caudal artery of Notch3WT and Notch3KO mice

ABSTRACT: Notch3 is a transmembrane receptor which is critically important for the structure and myogenic response of distal arteries, particularly cerebral arteries. After activation of the receptor, the intracellular domain translocates in the nucleus to activate target genes transcription. In order to identify Notch3 target genes, we used microarrays to compare gene expression from caudal arteries (Notch3+/+ vs Notch3-/-) and selected down-regulated genes in Notch3-/- arteries. Caudal arteries were dissected from 1 month old Notch3+/+ and Notch3-/- mice. RNA was prepared with arteries pooled from three (AJ_N3WT_3, AJ_N3WT_4) or four (AJ_N3WT_1, AJ_N3KO_1, AJ_N3KO_2, AJ_N3KO_4) mice and hybridized on Mouse MOE430.2 Affymetrix chips. For each group (Notch3+/+ and Notch3-/-), three biological replicates were performed.

ORGANISM(S): Mus musculus

SUBMITTER: Anne Joutel

PROVIDER: E-GEOD-36437 | biostudies-arrayexpress |

REPOSITORIES: biostudies-arrayexpress

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Transcriptome analysis for Notch3 target genes identifies Grip2 as a novel regulator of myogenic response in the cerebrovasculature.

Fouillade Charles C Baron-Menguy Céline C Domenga-Denier Valérie V Thibault Christelle C Takamiya Kogo K Huganir Richard R Joutel Anne A

Arteriosclerosis, thrombosis, and vascular biology 20121101 1

<h4>Objective</h4>Notch3 is critically important for the structure and myogenic response of distal arteries, particularly of cerebral arteries. However, signaling pathways acting downstream of Notch3 remain largely unknown.<h4>Methods and results</h4>Transcriptome analysis using tail arteries of Notch3-null mice identified a core set of 17 novel Notch3-regulated genes confirmed in tail or brain arteries. Postnatal deletion of RBP-Jκ in smooth muscle cells recapitulated the structural, functional ...[more]

PMID: 23117660

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Expression data from caudal artery of Notch3WT and Notch3KO mice

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