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Cytoplasmic Assembly and Selective Nuclear Import of Arabidopsis ARGONAUTE4/siRNA Complexes

ABSTRACT: Here we profiled small RNAs from whole cell, cytoplasmic and nuclear extracts from three-week-old Arabidopsis seedlings. We unexpectedly found that nuclear functional hc-siRNAs are predominantly present in the cytoplasm. Samples from Arabidopsis thaliana whole cell, cytoplasmic and nuclear extracts with 3 replicates for each. 9 samples in all.

ORGANISM(S): Arabidopsis thaliana

SUBMITTER: Yijun Qi

PROVIDER: E-GEOD-36500 | biostudies-arrayexpress |

REPOSITORIES: biostudies-arrayexpress

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Cytoplasmic assembly and selective nuclear import of Arabidopsis Argonaute4/siRNA complexes.

Ye Ruiqiang R Wang Wei W Iki Taichiro T Liu Chang C Wu Yang Y Ishikawa Masayuki M Zhou Xueping X Qi Yijun Y

Molecular cell 20120517 6

In plants, DNA methylation can be mediated by a class of Argonaute4 (AGO4)-associated heterochromatic siRNAs (hc-siRNAs), through a pathway termed RNA-directed DNA methylation (RdDM). It has been thought that RdDM is solely a nuclear process, as both the biogenesis and functioning of hc-siRNAs take place in the nucleus. In this study, we unexpectedly found that hc-siRNAs are predominantly present in the cytoplasm. We demonstrated that AGO4 is loaded with hc-siRNAs in the cytoplasm and the format ...[more]

PMID: 22608924

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