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P. pacificus young adults: Exposed to Staphylococcus aureus versus exposed to E. coli OP50 : 4 hours

ABSTRACT: Transcriptional profiling of P. pacificus young adult worms exposed to pathogen Staphylococcus aureus for 4 hours versus age-matched worms exposed to control lab food E. coli OP50. The goal was to identify genes regulated in response to pathogen. The broader goal of study was to study evolution of pathogen response by comparing this expression profile to that obtained by exposing the nematode C. elegans to the same pathogen. Other experiments which are a part of this study include expression profiling of C. elegans and P. pacificus on other pathogens including , Bacillus thuringiensis DB27, Serratia marcescens and Xenorhabdus nematophila. One-condition experiments. P. pacificus young adults: Exposed to Staphylococcus aureus versus exposed to E. coli OP50 : 4 hours. 3 biological replicates for each condition, including 2 dye-swaps.

ORGANISM(S): Pristionchus pacificus

SUBMITTER: Amit Sinha

PROVIDER: E-GEOD-36519 | biostudies-arrayexpress |

REPOSITORIES: biostudies-arrayexpress

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P. pacificus and C. elegans exposed to various pathogens

Project description:This SuperSeries is composed of the following subset Series: GSE36413 : C. elegans young adults : Exposed to Bacillus thuringiensis DB27 vs. E. coli OP50 exposure; 4hours GSE36493: C. elegans young adults: Exposed to Staphylococcus aureus versus exposed to E. coli OP50 : 4 hours GSE36499: C. elegans young adults: Exposed to Serratia marcescens versus exposed to E. coli OP50 : 4 hours GSE36501: C. elegans young adults: Exposed to Xenorhabdus nematophila versus exposed to E. coli OP50 : 4 hours GSE36517: P. pacificus young adults: Exposed to Bacillus thuringiensis DB27 versus exposed to E. coli OP50 : 4 hours GSE36519: P. pacificus young adults: Exposed to Staphylococcus aureus versus exposed to E. coli OP50 : 4 hours GSE36521: P. pacificus young adults: Exposed to Serratia marcescens versus exposed to E. coli OP50 : 4 hours GSE36523: P. pacificus young adults: Exposed to Xenorhabdus nematophila versus exposed to E. coli OP50 : 4 hours Refer to individual Series

2012-03-20 | E-GEOD-36636 | biostudies-arrayexpress

P. pacificus young adults: Exposed to Bacillus thuringiensis DB27 versus exposed to E. coli OP50 : 4 hours

Project description:Transcriptional profiling of P. pacificus young adult worms exposed to pathogen Xenorhabdus nematophila for 4 hours versus age-matched worms exposed to control lab food E. coli OP50. The goal was to identify genes regulated in response to pathogen. The broader goal of study was to study evolution of pathogen response by comparing this expression profile to that obtained by exposing the nematode C. elegans to the same pathogen. Other experiments which are a part of this study include expression profiling of C. elegans and P. pacificus on other pathogens including Staphylococcus aureus, Serratia marcescens and Xenorhabdus nematophila. One-condition experiments. P. pacificus young adults: Exposed to Bacillus thuringiensis DB27 versus exposed to E. coli OP50 : 4 hours. 4 biological replicates for each condition, including 2 dye-swaps.

2012-03-21 | E-GEOD-36517 | biostudies-arrayexpress

P. pacificus young adults: Exposed to Xenorhabdus nematophila versus exposed to E. coli OP50 : 4 hours

Project description:Transcriptional profiling of P. pacificus young adult worms exposed to pathogen Xenorhabdus nematophila for 4 hours versus age-matched worms exposed to control lab food E. coli OP50. The goal was to identify genes regulated in response to pathogen. The broader goal of study was to study evolution of pathogen response by comparing this expression profile to that obtained by exposing the nematode C. elegans to the same pathogen. Other experiments which are a part of this study include expression profiling of C. elegans and P. pacificus on other pathogens including Bacillus thuringiensis DB27, Staphylococcus aureus, and Serratia marcescens. One-condition experiments. P. pacificus young adults: Exposed to Xenorhabdus nematophila versus exposed to E. coli OP50 : 4 hours. 4 biological replicates for each condition, including 2 dye-swaps.

2012-03-21 | E-GEOD-36523 | biostudies-arrayexpress

P. pacificus young adults: Exposed to Serratia marcescens versus exposed to E. coli OP50 : 4 hours

Project description:Transcriptional profiling of P. pacificus young adult worms exposed to pathogen Serratia marcescens for 4 hours versus age-matched worms exposed to control lab food E. coli OP50. The goal was to identify genes regulated in response to pathogen. The broader goal of study was to study evolution of pathogen response by comparing this expression profile to that obtained by exposing the nematode C. elegans to the same pathogen. Other experiments which are a part of this study include expression profiling of C. elegans and P. pacificus on other pathogens including , Bacillus thuringiensis DB27, Serratia marcescens and Xenorhabdus nematophila. One-condition experiments. P. pacificus young adults: Exposed to Serratia marcescens versus exposed to E. coli OP50 : 4 hours. 4 biological replicates for each condition, including 2 dye-swaps.

2012-03-21 | E-GEOD-36521 | biostudies-arrayexpress

C. elegans young adults: Exposed to Bacillus thuringiensis DB27 versus exposed to E. coli OP50 : 4 hours

Project description:Transcriptional profiling of C. elegans young adult worms exposed to pathogen Bacillus thuringiensis DB27 for 4 hours versus age-matched worms exposed to onctrol lab food E. coli OP50. The goal was to identify genes regulated in response to pathogen. The broader goal of study was to study evolution of pathogen response by comparing this expression profile to that obtained by exposing the nematode Pristionchus pacificus to the same pathogen. Other experiments which are a part of this study include expression profiling of C. elegans and P. pacificus on other pathogens including Staphylococcus aureus, Serratia marcescens, Xenorhabdus nematophila. Keywords: Expression profiling by array One-condition experiments. C. elegans young adults: Exposed to Bacillus thuringiensis DB27 versus exposed to E. coli OP50 : 4 hours. 3 biological replicates for each condition, including 1 dye-swap.

2012-03-13 | E-GEOD-36413 | biostudies-arrayexpress

C. elegans young adults: Exposed to Serratia marcescens versus exposed to E. coli OP50 : 4 hours

Project description:Transcriptional profiling of C. elegans young adult worms exposed to pathogen Serratia marcescens for 4 hours versus age-matched worms exposed to control lab food E. coli OP50. The goal was to identify genes regulated in response to pathogen. The broader goal of study was to study evolution of pathogen response by comparing this expression profile to that obtained by exposing the nematode Pristionchus pacificus to the same pathogen. Other experiments which are a part of this study include expression profiling of C. elegans and P. pacificus on other pathogens including Bacillus thuringiensis, Staphylococcus aureus, and Xenorhabdus nematophila. One-condition experiments. C. elegans young adults: Exposed to Serratia marcescens versus exposed to E. coli OP50 : 4 hours. 4 biological replicates for each condition, including 2 dye-swaps.

2012-03-21 | E-GEOD-36499 | biostudies-arrayexpress

C. elegans young adults: Exposed to Xenorhabdus nematophila versus exposed to E. coli OP50 : 4 hours

Project description:Transcriptional profiling of C. elegans young adult worms exposed to pathogen Xenorhabdus nematophila for 4 hours versus age-matched worms exposed to control lab food E. coli OP50. The goal was to identify genes regulated in response to pathogen. The broader goal of study was to study evolution of pathogen response by comparing this expression profile to that obtained by exposing the nematode Pristionchus pacificus to the same pathogen. Other experiments which are a part of this study include expression profiling of C. elegans and P. pacificus on other pathogens including Bacillus thuringiensis, Staphylococcus aureus, and Serratia marcescens. One-condition experiments. C. elegans young adults: Exposed to Xenorhabdus nematophila versus exposed to E. coli OP50 : 4 hours. 4 biological replicates for each condition, including 2 dye-swaps.

2012-03-21 | E-GEOD-36501 | biostudies-arrayexpress

C. elegans young adults: Exposed to Staphylococcus aureus versus exposed to E. coli OP50 : 4 hours

Project description:Transcriptional profiling of C. elegans young adult worms exposed to pathogen Staphylococcus aureus for 4 hours versus age-matched worms exposed to onctrol lab food E. coli OP50. The goal was to identify genes regulated in response to pathogen. The broader goal of study was to study evolution of pathogen response by comparing this expression profile to that obtained by exposing the nematode Pristionchus pacificus to the same pathogen. Other experiments which are a part of this study include expression profiling of C. elegans and P. pacificus on other pathogens including Staphylococcus aureus, Serratia marcescens, Xenorhabdus nematophila. Keywords: Expression profiling by array One-condition experiments. C. elegans young adults: Exposed to Staphylococcus aureus versus exposed to E. coli OP50 : 4 hours. 4 biological replicates for each condition, including 2 dye-swaps.

2012-03-21 | E-GEOD-36493 | biostudies-arrayexpress

Immune reposne of germline-ablated P. pacificus exposed to S. marcescens

Project description:Transcriptional profiling of germline-ablated P. pacificus worms exposed to S. marcescens for 8 hours versus germline-ablated P. pacificus exposed to the control E. coli OP50. First goal was to identify genes that are involved in immune response of germline-ablated P. pacificus when exposed to S. marcescens. Second, this was compared to longevity-regulating genes in germline-deficient animals (see series GSE37331). One-condition experiments. Germline ablated P. pacificus worms exposed to S. marcescens for 8 hours versus germline-ablated P. pacificus exposed to the control E. coli OP50. 4 biological replicates for each condition, including 2 dye-swaps.

2012-04-17 | E-GEOD-37337 | biostudies-arrayexpress

Effect of germline ablation in the nematode P. pacificus

Project description:Transcriptional profiling of germline-ablated P. pacificus worms versus un-ablated wild-type controls. Food source E. coli OP50 for both conditions. The goal was to identify genes that regulate the enhanced longevity observed in germline-deficient animals. One-condition experiments. Germline ablated P. pacificus versus un-ablated wild-type P. pacificus. Developmental stage = Young adults. Food source = E. coli OP50 for both conditions. 3 biological replicates for each condition, including 2 dye-swaps.

2012-04-17 | E-GEOD-37331 | biostudies-arrayexpress

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