Polycomb Cbx orthologs mediate the balance between Hematopoietic Stem Cell self-renewal and differentiation

ABSTRACT: By genome-wide ChIP-seq analyses we show that Cbx7 and Cbx8 share ~95% of their targets in bone marrow cells. Cbx7 and Cbx8 have only ~200 targets that are not overlapping between the two proteins. These distinct Cbx7 and Cbx8 targets show reciprocal expression patterns along hematopoietic differentiation. Overexpression of empty vector (negative control), flag-Cbx7 and flag-Cbx8 in 5-FU treated bone marrow cells (progenitor and stem cells), followed by 1 week culture and subsequent ChIP using flag-M2 agarose beads.

ORGANISM(S): Mus musculus

SUBMITTER: Leonid Bystrykh 

PROVIDER: E-GEOD-36658 | biostudies-arrayexpress |

REPOSITORIES: biostudies-arrayexpress