Unknown,Transcriptomics,Genomics,Proteomics

Dataset Information

Genome wide binding sites of Smad3 and JMJD3 in neural stem cells

ABSTRACT: Neural development requires crosstalk between signaling pathways and chromatin. In this study, we demonstrate that neurogenesis is promoted by an interplay between the TGFM-NM-2 pathway and the H3K27me3 histone demethylase (HDM) JMJD3. Genome-wide analysis showed that JMJD3 is targeted to gene promoters by Smad3 in neural stem cells (NSCs) and is essential to activate TGFM-NM-2-responsive genes. In vivo experiments in chick spinal cord revealed that the generation of neurons promoted by Smad3 is dependent on JMJD3 HDM activity. Overall, these findings indicate that JMJD3 function is required for the TGFM-NM-2 developmental program to proceed. We immunoprecipitate endogenous Smad3 or JMJD3 proteins from neural stem cells treated with TGFb for 30 minutes.

ORGANISM(S): Mus musculus

SUBMITTER: Conchi EI

PROVIDER: E-GEOD-36673 | biostudies-arrayexpress |

REPOSITORIES: biostudies-arrayexpress

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Genome-wide analysis reveals that Smad3 and JMJD3 HDM co-activate the neural developmental program.

Estarás Conchi C Akizu Naiara N García Alejandra A Beltrán Sergi S de la Cruz Xavier X Martínez-Balbás Marian A MA

Development (Cambridge, England) 20120801 15

Neural development requires crosstalk between signaling pathways and chromatin. In this study, we demonstrate that neurogenesis is promoted by an interplay between the TGFβ pathway and the H3K27me3 histone demethylase (HDM) JMJD3. Genome-wide analysis showed that JMJD3 is targeted to gene promoters by Smad3 in neural stem cells (NSCs) and is essential to activate TGFβ-responsive genes. In vivo experiments in chick spinal cord revealed that the generation of neurons promoted by Smad3 is dependent ...[more]

PMID: 22782721

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Dataset Information

Genome wide binding sites of Smad3 and JMJD3 in neural stem cells

Publications

Genome-wide analysis reveals that Smad3 and JMJD3 HDM co-activate the neural developmental program.

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