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The global gene expression profile of gain of function miR34c in osteoblast

ABSTRACT: To examine the unbiased global gene expression of gain of miR34c in committed osteoblast in mouse we have performed expression microarray. This approach will allow us to define the novel targets that are negatively regulated by over-expression of miR34c in osteoblast. Total RNA was isolated from primary calvaria from WT and GOF miR34c mouse (n=3) at the age of P3. WT primary calvarial samples were served as control.

ORGANISM(S): Mus musculus

SUBMITTER: Yangjin Bae

PROVIDER: E-GEOD-36780 | biostudies-arrayexpress |

REPOSITORIES: biostudies-arrayexpress

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miRNA-34c regulates Notch signaling during bone development.

Bae Yangjin Y Yang Tao T Zeng Huan-Chang HC Campeau Philippe M PM Chen Yuqing Y Bertin Terry T Dawson Brian C BC Munivez Elda E Tao Jianning J Lee Brendan H BH

Human molecular genetics 20120412 13

During bone homeostasis, osteoblast and osteoclast differentiation is coupled and regulated by multiple signaling pathways and their downstream transcription factors. Here, we show that microRNA 34 (miR-34) is significantly induced by BMP2 during osteoblast differentiation. In vivo, osteoblast-specific gain of miR-34c in mice leads to an age-dependent osteoporosis due to the defective mineralization and proliferation of osteoblasts and increased osteoclastogenesis. In osteoblasts, miR-34c target ...[more]

PMID: 22498974

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