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Genome-wide analysis of Crohn's disease and ulcerative colitis biopsy samples.

ABSTRACT: Gene expression patterns of Crohn's disease (CD) and ulcerative colitis (UC) colonic specimens were analyzed using whole-genome microarrays. Healthy control samples were included in order to detect gene expression changes associated with CD or UC. CD and UC samples were also compared in order to identify the molecular mechanisms that distinguish both fenotypes of inflammatory bowel disease. Total RNA obtained from intestinal biopsies were analyzed using whole-genome microarrays.

ORGANISM(S): Homo sapiens

SUBMITTER: Trinidad Montero

PROVIDER: E-GEOD-36807 | biostudies-arrayexpress |

REPOSITORIES: biostudies-arrayexpress

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Genome-wide analysis of Crohn's disease and ulcerative colitis biopsy samples.

Project description:Gene expression patterns of Crohn's disease (CD) and ulcerative colitis (UC) colonic specimens were analyzed using whole-genome microarrays. Healthy control samples were included in order to detect gene expression changes associated with CD or UC. CD and UC samples were also compared in order to identify the molecular mechanisms that distinguish both fenotypes of inflammatory bowel disease.

2013-09-23 | GSE36807 | GEO

MicroRNA expression in human colon: Crohn's disease versus ulcerative colitis

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Gene expression analysis of omental and mesenteric adipose tissue in Crohn's disease and ulcerative colitis

Project description:Our hypothesis is that in IBD patients intestinal bacteria translocation into the intra-abdominal fat depots affects adipocyte morphology and gene expression. The study aimed to study adipocyte gene expression of omental (OM) and mesenteric (MES) adipose tissue of ulcerative colitis (UC) and crohn's disease (CD). Total RNA was extracted from isolated adipocytes from omental and mesenteric adipose tissue of CD and UC patients. Microarray experiments were performed in duplicates of 4 different pools of RNAs extracted from adipocytes isolated from OM and MES of UC patients (n=5) and CD patients (n=5) respectively.

2013-05-09 | E-GEOD-46754 | biostudies-arrayexpress

Transcription profiling of peripheral blood samples from patients with Crohn's disease (CD) and Ulcerative colitis (UC) .

Project description:The whole-genome oligonucleotide microarray analysis of peripheral blood samples can contribute to the determination of distant blood markers of local pathophysiological alterations in colorectal diseases. These markers can lead to alternative screening procedures. Total RNA was extracted from peripheral blood samples of patients with Ulcerative colitis (UC) and Crohn's disease (CD) and hybridized on Affymetrix HGU133 Plus 2.0 microarrays

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Transcription profiling of CD8 T-cells predicts disease course in Crohn's disease and ulcerative colitis

Project description:The natural history of Crohn's disease (CD) and ulcerative colitis (UC) is variable. In patients with frequently-relapsing/steroid-refractory disease, sustained remission is more likely if immunomodulators are introduced earlier. However, many patients experience quiescent disease without immunomodulators, and should not be unnecessarily immunosuppressed. Previous attempts to stratify treatment from diagnosis have failed because disease course cannot be reliably predicted. We performed whole genome expression analysis of peripheral CD8 and CD4 T-cells isolated from patients with active, untreated CD or UC. Patients were prospectively managed by clinicians blinded to the microarray results. Analysis of CD8 T-cell gene-expression data revealed two distinct subgroups within each disease cohort, which did not correlate with any contemporaneous laboratory or clinical data. There was considerable overlap in the genes that were differentially expressed between the subgroups in each disease. Patients belonging to one of the subgroups (termed IBD1) experienced significantly more aggressive disease than those in the other (termed IBD2) in both diseases. This was characterised by a greater need to escalate treatment with immunomodulators or surgery in response to relapsing or chronically active disease. Furthermore, this subgroup more frequently required additional interventions due to the initial escalation failing to maintain disease remission. Similar association with disease behaviour could not be demonstrated with CD4 T-cell gene-expression analysis, nor with previously described methods of predicting outcome. Our findings demonstrate that gene-expression profiling of CD8 T-cells at diagnosis can stratify patients with CD or UC according to subsequent disease behaviour and represents a significant step towards personalised therapy.

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Gene expression analysis of omental and mesenteric adipose tissue in Crohn's disease and ulcerative colitis

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Metabonomics of human fecal extracts characterize ulcerative colitis, Crohn's disease and healthy individuals

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Differential pathogenic Th17 profile in mesenteric lymph nodes of Crohn's disease and ulcerative colitis patients

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Short Noncoding RNAs as predictive biomarkers for the development from Inflammatory Bowel Disease Unclassified to Crohn’s Disease or Ulcerative Colitis

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Gut biopsies from patients with Crohn's Disease and Ulcerative Colitis and healthy controls

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