Unknown,Transcriptomics,Genomics,Proteomics

Dataset Information

0

Elevating Sox2 levels deleteriously affects the growth of glioblastoma and medulloblastoma cells.


ABSTRACT: Induction of the transcription factor Sox2 from a doxycycline-inducible promoter in iSox2-DAOY medulloblastoma cells. Affymetrix microarrays were used to characterize the gene expression profile of iSox2-DAOY cells in the absence and presence of doxycycline. Human DAOY medulloblastoma cells were engineered to express epitope tagged Sox2 under the control of a doxycycline inducible promoter, to produce iSox2-DAOY cells. iSox2-DAOY cells were FACS sorted into CD133 low and high populations. The CD133 low population was cultured in the absence and presence of 0.5 µg/mL doxycycline for 24 hours. RNA was extracted from cells cultured without and with doxycycline, and was used for microarray analysis.

ORGANISM(S): Homo sapiens

SUBMITTER: Angie Rizzinio 

PROVIDER: E-GEOD-36947 | biostudies-arrayexpress |

REPOSITORIES: biostudies-arrayexpress

Similar Datasets

2012-02-20 | E-GEOD-34801 | biostudies-arrayexpress
2012-08-25 | GSE36947 | GEO
2022-03-14 | PXD030597 | Pride
2022-03-03 | PXD029660 | Pride
2007-10-31 | E-GEOD-7578 | biostudies-arrayexpress
2014-07-31 | E-GEOD-46045 | biostudies-arrayexpress
2022-08-04 | PXD031870 | Pride
2016-02-17 | E-GEOD-77947 | biostudies-arrayexpress
2022-03-02 | E-MTAB-9823 | biostudies-arrayexpress
2023-06-28 | E-MTAB-11914 | biostudies-arrayexpress