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Yeast genomic expression patterns in response to type B trichothecenes

ABSTRACT: The evaluation of mycotoxicity of type B trichothecenes using a yeast gene expression comparison analysis. The yeast BY4743 derivative PDR5 mutant was used for this study. The yeast cells were treated with trichothecene mycotoxins, and incubated at 25 degree for two hours, respectively. Total RNA was isolated with commercial kit (FastRNA Pro Red kit, Q-Biogene), and amplified RNA (aRNA) was synthesized with 3'IVT Express kit (Affymetrix). All samples were hybridized with Gene Chip (Yeast Genome 2.0 Array, Affymetrix), then each array chip was scanned by GeneChip Sanner 3000 (Affymetrix). The yeast strain was grown in YPD medium with 25 ppm of trichothecene mycotoxin (deoxynivalenol (DON), 3-acetyldeoxynivalenol (3ADON), 15-acetyldeoxynivalenol (15ADON), nivalenol (NIV), or fusarenone-X (FusX)). The same volume of DMSO was added to the control samples. The conditioned samples were normalized with three replicates.

ORGANISM(S): Saccharomyces cerevisiae

SUBMITTER: Tadahiro Suzuki

PROVIDER: E-GEOD-36954 | biostudies-arrayexpress |

REPOSITORIES: biostudies-arrayexpress

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Comprehensive gene expression analysis of type B trichothecenes.

Suzuki Tadahiro T Iwahashi Yumiko Y

Journal of agricultural and food chemistry 20120907 37

Type B trichothecenes, deoxynivalenol (DON) and nivalenol (NIV), are secondary metabolites of Fusarium species and are major pollutants in food and feed products. Recently, the production trend of their derivatives, 3-acetyldeoxynivalenol (3-AcDON), 15-acetyldeoxynivalenol (15-AcDON), and 4-acetylnivalenol (4-AcNIV or fusarenon-X), has been changing in various regions worldwide. Although in vivo behavior has been reported, it is necessary to acquire more detailed information about these deriva ...[more]

PMID: 22897823

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