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Expression data from 11-day-old antisense BoEMF2.1 broccoli

ABSTRACT: EMF2 genes play an important function in inhibiting reproductive programs during vegetative stage of plant development. We knocked down the expression of endogenous EMF2 genes in broccoli by introduing a 35S::antisenseBoEMF2.1 contruct. The transgenic broccoli with knocking-down expression of endogenous EMF2s showed aberrant phenotypes during development. We used microarrays to study the global programme of gene expression and to identified genes misexpressed in broccoli with knocking-down expression of endogenous EMF2 genes 11-day-old seedlings of broccoli harboring the empty vector pCAMBIA1380 and that harboring 35S::antisenseBoEMF2.1 (asBoEMF2.1) were used for RNA extraction and hybridization on Affymetrix microarrays.

ORGANISM(S): Brassica oleracea var. italica

SUBMITTER: Mao-Sen Liu

PROVIDER: E-GEOD-36963 | biostudies-arrayexpress |

REPOSITORIES: biostudies-arrayexpress

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Expression data from 11-day-old antisense BoEMF2.1 broccoli

Project description:EMF2 genes play an important function in inhibiting reproductive programs during vegetative stage of plant development. We knocked down the expression of endogenous EMF2 genes in broccoli by introduing a 35S::antisenseBoEMF2.1 contruct. The transgenic broccoli with knocking-down expression of endogenous EMF2s showed aberrant phenotypes during development. We used microarrays to study the global programme of gene expression and to identified genes misexpressed in broccoli with knocking-down expression of endogenous EMF2 genes

2012-03-31 | GSE36963 | GEO

Expression data from 7-day-old Arabidopsis emf2 mutant, rescued emf2 mutant harboring 35S::BoEMF2.1, WT Columbia ecotype and WT harboring 35S::BoEMF2.1 named transWT

Project description:Arabidopsis emf2 mutants bypass vegetative development and flowering upon seed germination. We introduced a broccoli BoEMF2.1 gene into emf2 mutants and obtained rescued emf2 plants that harbored 35S::BoEMF2.1. We found that BoEMF2.1 can partially rescue the phenotype of emf2 to that of WT. We used microarrays to study the global program of gene expression and to identify genes misexpressed in the Arabidopsis emf2 mutant that had been rescued by 35S::BoEMF2.1. 7-day-old seedlings of WT, transWT, emf2 and rescued emf2 Arabidopsis were used for RNA extraction and hybridization on Affymetrix microarrays.

2012-03-29 | E-GEOD-36943 | biostudies-arrayexpress

Expression data from 7-day-old Arabidopsis emf2 mutant, rescued emf2 mutant harboring 35S::BoEMF2.1, WT Columbia ecotype and WT harboring 35S::BoEMF2.1 named transWT

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Transcription profiling of human colon Caco2 cells treated with extracts from broccoli that had been cooked for different lengths of time

Project description:Caco2 cells were treated 24 hours with broccoli extracts that had been cooked for different lengths of time.

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Identification of miRNAs-seq of 4-day broccoli sprouts and putative cross-kingdom regulated human target genes

Project description:We want to obtained miRNA-seq from 4 day-old broccoli sprouts by deep sequencing and identified the binding sites of the those miRNAs in human target genes. Meanwhile, we extracted the human target genes from published paper that were regulated by broccoli and compared those genes with the predicted human targets of 4-day broccoli sprouts.

2020-02-01 | E-MTAB-7155 | biostudies-arrayexpress

Arabidopsis thaliana

Project description:Expression data from 7-day-old Arabidopsis emf2 mutant, rescued emf2 mutant harboring 35S::BoEMF2.1, WT Columbia ecotype and WT harboring 35S::BoEMF2.1 named transWT

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Transcriptional analysis of exogenously supplied and transgenic of senescence-induced cytokinin for broccoli postharvest yellowing improvement

Project description:Both exogenously supplied and transgenic induced cytokinin production can effectively delay senescence of broccoli florets during postharvest storage. However, a substantial comparison between the mechanisms of these two treatments on delaying broccoli florets senescence was absent. Here, we conduct microarray analysis on broccoli florets of N6-benzylaminopurine treated and ipt-transgenic broccoli that harbor a senescence-associated-gene promoter triggering isopentenyltransferase gene expression during postharvest storage. Analysis used RNA of Green King inbred line 104 as control sample for comparison to the experimental samples of ipt-transgenic line 102, 103 and parental line Green King as well as 10 ppm BA treated Green King at harvest and after postharvest storage at 25 centigrade in the dark for 4 days.

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Systematic Discovery of Deeply Conserved Plan Protein Complexes, Broccoli leaves

Project description:The associated files are mass spec data from individual fractions of mixed-bed ion exchange fractionations of native extract made from broccoli plant leaves (Brassica oleracea var. italica).

2019-10-28 | PXD013282 | Pride

Systematic Discovery of Deeply Conserved Plant Protein Complexes, Broccoli nucleii, isoelectric focusing

Project description:The associated files are mass spec data from native nuclear extract from broccoli florets (Brassica oleracea var. italica)fractionated on an Agilent 3100 OFFGEL isoelectric focusing apparatus.

2019-10-28 | PXD013281 | Pride

Systematic Discovery of Deeply Conserved Plant Protein Complexes, Broccoli Nuclei

Project description:The associated files are mass spec data from individual fractions of ion exchange or size exclusion fractionations of native nuclear extract made from broccoli florets (Brassica oleracea var. italica). Ion exchange chromatography was on a concatenated WAX-WAX-CAT column series.

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