Unknown,Transcriptomics,Genomics,Proteomics

Dataset Information

Expression data from gbf1, gbf1 hy5, and gbf1 hyh mutants

ABSTRACT: Three bZIP transcription factors (TFs), namely GBF1, HY5, and HYH, form heterodimers with each other and regulate photomorphogenesis in an interdependent manner. GBF1 acts as both a positive and negative regulator of photomorphogenesis, whereas HY5 and HYH mainly act as positive regulators of photomorphogenesis. To study the effect of HY5 and HYH on GBF1-mediated genome-wide expression, transcript profiling was performed in the gbf1 single mutant and gbf1hy5 and gbf1hyh double mutants. Our study revealed that GBF1 mainly acts antagonistic to HY5 and HYH for genome-wide expression. Four-day-old constant white-light grown Arabidipsis seedlings of wild-type (Col-0), gbf1 mutant, gbf1 hy5 double mutant and gbf1 hyh double mutant were harvested for total RNA extraction and Affymetrix microarray hybridisation. Two biological replicates of each sample were used for hybridisation and data analysis. Data were analysed using GeneSpring software by utilising the RMA algorithm. To draw the conclusions from this study, probe level intensity of gbf1, gbf1 hy5, and gbf1 hyh was normalised against the probe level intensity of wild-type, and also the probe level intensity of gbf1 hy5 and gbf1 hyh double mutants was normalised against the probe level intensity of the gbf1 single mutant.

ORGANISM(S): Arabidopsis thaliana

SUBMITTER: Sudip Chattopadhyay

PROVIDER: E-GEOD-36964 | biostudies-arrayexpress |

REPOSITORIES: biostudies-arrayexpress

ACCESS DATA

Similar Datasets

Project description:Exposure to high irradiance results in dramatic changes in nuclear gene expression in plants. However, little is known about the mechanisms by which changes in irradiance are sensed and how the information is transduced to the nucleus to initiate the genetic response. To investigate whether the photoreceptors are involved in the response to high irradiance, we analyzed expression of ELIP1, ELIP2, APX2 and LHCB2.4 in the phyA, phyB, cry1 and cry2 photoreceptor mutants and hy5 and hyh transcription factor mutants. Following exposure to high intensity white light for 3 h (HL, 1000 micro mol quanta m-2 s-1) expression of ELIP1/2 and APX2 was strongly induced and LHCB2.4 expression repressed in wild type. The cry1 and hy5 mutants showed specific mis-regulation of ELIP1/2 and we show that the induction of ELIP1/2 expression is mediated via CRY1 in a blue light intensity-dependent manner. Furthermore, using the Affymetrix Arabidopsis 24K Gene-Chip we showed that 77 of the HL responsive genes are regulated via CRY1, and 26 of those genes were also HY5 dependent. As a consequence of the mis-regulation of these genes the cry1 mutant displayed a high irradiance-sensitive phenotype with significant photoinactivation of PSII, indicated by reduced Fv/Fm. Thus, we describe a novel function of CRY1 in mediating plant responses to high irradiances that is essential to the induction of photoprotective mechanisms. This indicates that high irradiance can be sensed in a chloroplast-independent manner by a cytosolic/nucleic component. Experiment Overall Design: All samples were done in biological triplicates (named A, B, C): Arabidopsis Col-O: growth conditions (7 d continuous white light; 100 micro mol quanta m-2 s-1, 22 Â°C), growth conditions followed by high light (3 hours 1000 micro mol quanta m-2 s-1, 22 Â°C) and growth conditions followed by blue light (3 hours; HQI-T 400 W lamps were filtered through color filter #74, 400-540 nm, absorption maximum: 470 nm (Night Blue; Rosco International)) Experiment Overall Design: cryptochrome 1 (cry1) mutant: growth conditions and growth conditions followed by 3 hours high light Experiment Overall Design: long hypocotyl 5 (hy5) mutant: growth conditions and growth conditions followed by 3 hours high light

Dataset Information

Expression data from gbf1, gbf1 hy5, and gbf1 hyh mutants

Similar Datasets

OmicsDI is part of the ELIXIR infrastructure

Tweets