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Gene expression patterns of distinct human endothelial precursor cells

ABSTRACT: Early EPCs (eEPCs) appear at less than 1 week in culture dishes, whereas late EPCs (LEPCs) appear late at 2-4 weeks. Distinct angiogenic properties between these two EPC subpopulations have been disclosed by the angiogenesis assay: late EPCs, but not eEPCs, form vascular networks de novo and are able to incorporate into vascular networks. On the contrary, eEPCs, but not late ones, indirectly augment tubulogenesis even when physically separated by a Transwell membrane, implying the involvement of a cytokine-based paracrine mechanism. Microarrays are great tools to M-bM-^@M-^\snapshotM-bM-^@M-^] the transcriptome profiles, and thus provide deeper insights into gene expressions and microRNA-gene interactions. Cord blood mononuclear cells (MNCs) were Histopaque-1077 isolated and plated in endothelial growth medium-2 on fibronectin-coated six-well plates at 37M-BM-0C. After 3 days of culturing, attached eEPCs appeared, and medium and nonadherent cells were removed. Thereafter, the medium were replaced every 2 days, and a certain number of eEPCs continued to grow into the LEPCs colonies, which emerge 2M-bM-^@M-^S4 weeks after the start of MNC culture. Total RNA of early and late EPCs were extracted and evaluated with Affymetrix GeneChip Human U133 plus 2.0

ORGANISM(S): Homo sapiens

SUBMITTER: Tse-Shun Huang

PROVIDER: E-GEOD-37045 | biostudies-arrayexpress |

REPOSITORIES: biostudies-arrayexpress

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Gene expression patterns of distinct human endothelial precursor cells

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Expression data from human healthy EPCs/CACs

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2011-04-13 | E-GEOD-26949 | biostudies-arrayexpress

Expression data from human healthy and lupus EPCs/CACs, and healthy CD133+ bone marrow EPCs

Project description:Systemic lupus erythematosus (SLE) is characterized by increased vascular risk due to premature atherosclerosis independent of traditional risk factors. We previously proposed that interferon-α plays a crucial role in premature vascular damage in SLE. IFN-α alters the balance between endothelial cell apoptosis and vascular repair mediated by endothelial progenitor cells (EPCs) and myeloid circulating angiogenic cells (CACs). Here we demonstrate that IFN-α promotes an antiangiogenic signature in SLE and control EPCs/CACs, characterized by transcriptional repression of IL-1α and β, IL-1 receptor 1 and vascular endothelial growth factor A (VEGF-A) and upregulation of IL-1 receptor antagonist (IL-1RN) and the decoy receptor IL1-R2. IL-1β promotes significant improvement in the functional capacity of lupus EPCs/CACs, therefore abrogating the deleterious effects of IFN-α. We used microarrays to analyze the effect of IFNα on peripheral blood EPCs/CACs and on bone marrow EPCs exposed to proangiogenic stimulation. This SuperSeries is composed of the SubSeries listed below. Human healthy and lupus EPCs and CACs from PBMCs, and healthy EPCs from bone marrow, were isolated and cultured under proangiogenic stimulation; after IFN-α incubation or not, RNA was extracted and processed for hybridization on Affymetrix microarrays.

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