Unknown,Transcriptomics,Genomics,Proteomics

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RNA-sequencing analysis of NB4 cells overexpressing miR-125b


ABSTRACT: To better understand the mechanisms of blockage of myeloid differentiation and apoptosis and induction of proliferation by miR-125b, we proceeded to identify miR-125b target genes involved in these pathways. We analyzed the total cellular gene expression pattern by RNA-sequencing of the parental NB4 myeloid cell line and that transiently transfected with miR-125b. We generated four cDNA libraries corresponding to duplicates of miR-125b and control cells. Compare the gene expression levels in miR control transfected cells with that in miR-125b transfected NB4 cells.

ORGANISM(S): Homo sapiens

SUBMITTER: Diu Nguyen 

PROVIDER: E-GEOD-37061 | biostudies-arrayexpress |

REPOSITORIES: biostudies-arrayexpress

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Publications

MicroRNA-125b transforms myeloid cell lines by repressing multiple mRNA.

Bousquet Marina M   Nguyen Diu D   Chen Cynthia C   Shields Lauren L   Lodish Harvey F HF  

Haematologica 20120611 11


<h4>Background</h4>We previously described a t(2;11)(p21;q23) chromosomal translocation found in patients with myelodysplasia or acute myeloid leukemia that leads to over-expression of the microRNA miR-125b, and we showed that transplantation of mice with murine stem/progenitor cells overexpressing miR-125b is able to induce leukemia. In this study, we investigated the mechanism of myeloid transformation by miR-125b.<h4>Design and methods</h4>To investigate the consequences of miR-125b over-expr  ...[more]

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