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Transcription profiling of wild type and H1c, H1d, H1e triple null mouse embryonic stem cells to study the effects of loss of the three linker histone subtypes on gene regulation.

ABSTRACT: Affymetrix expression profiling of wild-type and H1c, H1d, H1e triple null mouse embryonic stem cells revealed that loss of three linker histone subtypes (H1c, H1d and H1e) results in specific changes in gene regulation. Experiment Overall Design: Affymetrix experiments performed on RNA extracted from wild-type and two H1c/H1d/H1e triple null ES cell lines and data acquired in duplicate.

ORGANISM(S): Mus musculus

SUBMITTER: Yuhong Fan

PROVIDER: E-GEOD-3714 | biostudies-arrayexpress |

REPOSITORIES: biostudies-arrayexpress

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Histone H1 depletion in mammals alters global chromatin structure but causes specific changes in gene regulation.

Fan Yuhong Y Nikitina Tatiana T Zhao Jie J Fleury Tomara J TJ Bhattacharyya Riddhi R Bouhassira Eric E EE Stein Arnold A Woodcock Christopher L CL Skoultchi Arthur I AI

Cell 20051201 7

Linker histone H1 plays an important role in chromatin folding in vitro. To study the role of H1 in vivo, mouse embryonic stem cells null for three H1 genes were derived and were found to have 50% of the normal level of H1. H1 depletion caused dramatic chromatin structure changes, including decreased global nucleosome spacing, reduced local chromatin compaction, and decreases in certain core histone modifications. Surprisingly, however, microarray analysis revealed that expression of only a smal ...[more]

PMID: 16377562

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Expression data from WT, H1c/H1d/H1e triple null ES cells

Project description:Affymetrix expression profiling of wild-type and H1c, H1d, H1e triple null mouse embryonic stem cells revealed that loss of three linker histone subtypes (H1c, H1d and H1e) results in specific changes in gene regulation. Keywords: cell comparison

2006-01-15 | GSE3714 | GEO

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Gene expression in Histone H1 null mutant cells

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gene activation by IFNs in wild type and Pias1 null BMMs

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