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S-phase time-course of Lachancea kluyveri cells

ABSTRACT: Haploid cells of Lachancea kluyveri were arrested in G1 phase using Saccharomices cerevisiae alpha factor. After, release in a new media, cells go synchronously through S-phase. One sample is taken every five minutes. Microarrays are used to monitor the change of DNA copy number from 1 to 2, all along the genome during S-phase. Two-condition experiment, G1 cells vs. S-phase cells at different time points. Biological replicates: 3 biological replicates.

ORGANISM(S): Lachancea kluyveri

SUBMITTER: AGIER nicolas 

PROVIDER: E-GEOD-37244 | biostudies-arrayexpress |

REPOSITORIES: biostudies-arrayexpress

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The spatiotemporal program of replication in the genome of Lachancea kluyveri.

Agier Nicolas N   Romano Orso Maria OM   Touzain Fabrice F   Cosentino Lagomarsino Marco M   Fischer Gilles G  

Genome biology and evolution 20130101 2

We generated a genome-wide replication profile in the genome of Lachancea kluyveri and assessed the relationship between replication and base composition. This species diverged from Saccharomyces cerevisiae before the ancestral whole genome duplication. The genome comprises eight chromosomes among which a chromosomal arm of 1 Mb has a G + C-content much higher than the rest of the genome. We identified 252 active replication origins in L. kluyveri and found considerable divergence in origin loca  ...[more]

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