Base-pair resolution DNA methylation sequencing reveals profoundly divergent epigenetic landscapes in Acute Myeloid Leukemia
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ABSTRACT:
ORGANISM(S): Homo sapiens
SUBMITTER: Altuna Akalin
PROVIDER: E-GEOD-37454 | biostudies-arrayexpress |
REPOSITORIES: biostudies-arrayexpress
Publications
Base-pair resolution DNA methylation sequencing reveals profoundly divergent epigenetic landscapes in acute myeloid leukemia.
Akalin Altuna A Garrett-Bakelman Francine E FE Kormaksson Matthias M Busuttil Jennifer J Zhang Lu L Khrebtukova Irina I Milne Thomas A TA Huang Yongsheng Y Biswas Debabrata D Hess Jay L JL Allis C David CD Roeder Robert G RG Valk Peter J M PJ Löwenberg Bob B Delwel Ruud R Fernandez Hugo F HF Paietta Elisabeth E Tallman Martin S MS Schroth Gary P GP Mason Christopher E CE Melnick Ari A Figueroa Maria E ME
PLoS genetics 20120621 6
We have developed an enhanced form of reduced representation bisulfite sequencing with extended genomic coverage, which resulted in greater capture of DNA methylation information of regions lying outside of traditional CpG islands. Applying this method to primary human bone marrow specimens from patients with Acute Myelogeneous Leukemia (AML), we demonstrated that genetically distinct AML subtypes display diametrically opposed DNA methylation patterns. As compared to normal controls, we observed ...[more]
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