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MiRNA expression analysis of cancer-associated fibroblasts (CAFs) and normal fibroblasts (NFs) in breast cancer

ABSTRACT: To identify miRNA differentially expressed in CAFs vs matched NFs and analyze the heterogeneity of miRNA expression profiles in the two kinds of cells, we established primary cultures of CAFs and paired NFs from six resected breast tumor tissue without any radiotherapy and chemotherapy treatment. Paired CAFs and NFs from six primary human breast carcinoma specimens were cultured,the third passage of primary cells was used in the experiments.

ORGANISM(S): Homo sapiens

SUBMITTER: liuyang zhao

PROVIDER: E-GEOD-37527 | biostudies-arrayexpress |

REPOSITORIES: biostudies-arrayexpress

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MiRNA expression analysis of cancer-associated fibroblasts and normal fibroblasts in breast cancer.

Zhao Liuyang L Sun Yan Y Hou Yixuan Y Peng Qiongle Q Wang Liyang L Luo Haojun H Tang Xi X Zeng Zongyue Z Liu Manran M

The international journal of biochemistry & cell biology 20120813 11

Cancer-associated fibroblasts (CAFs) promote tumorigenesis, growth, invasion and metastasis of cancer, whereas normal fibroblasts (NFs) are thought to suppress tumor progression. Little is known about miRNAs expression differences between CAFs and NFs or the patient-to-patient variability in miRNAs expression in breast cancer. We established primary cultures of CAFs and paired NFs from six resected breast tumor tissues that had not previously received radiotherapy or chemotherapy treatment and a ...[more]

PMID: 22964023

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