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U937 scramble vs shHDAC2 upon SAHA (Vorinostat; suberoylanilide hydroxamic acid) treatment

ABSTRACT: Transcriptional profiling of U937 scramble vs shHDAC2 before and after SAHA treatment at 5ÂµM concentration for 6 and 24 hours. Different Experimental Conditions: U937 scramble (U937 trasfected with empty vector) vs shHDAC2 (U937 trasfected with shHDAC2 vector), untreated and treated with SAHA at 5 ÂµM concentration for 6h and 24h. Biological replicates: 2 for each sample, independently grown and harvested at 6 and 24 hours. One replicate per array.

ORGANISM(S): Homo sapiens

SUBMITTER: Mariarosaria Conte

PROVIDER: E-GEOD-37529 | biostudies-arrayexpress |

REPOSITORIES: biostudies-arrayexpress

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HDAC2 deregulation in tumorigenesis is causally connected to repression of immune modulation and defense escape.

Conte Mariarosaria M Dell'Aversana Carmela C Benedetti Rosaria R Petraglia Francesca F Carissimo Annamaria A Petrizzi Valeria Belsito VB D'Arco Alfonso Maria AM Abbondanza Ciro C Nebbioso Angela A Altucci Lucia L

Oncotarget 20150101 2

Histone deacetylase 2 (HDAC2) is overexpressed or mutated in several disorders such as hematological cancers, and plays a critical role in transcriptional regulation, cell cycle progression and developmental processes. Here, we performed comparative transcriptome analyses in acute myeloid leukemia to investigate the biological implications of HDAC2 silencing versus its enzymatic inhibition using epigenetic-based drug(s). By gene expression analysis of HDAC2-silenced vs wild-type cells, we found ...[more]

PMID: 25473896

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