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Effect of small molecules on activated astrocytes

ABSTRACT: We have developed an assay to test the neuroprotective properties of compounds using stem cellM-bM-^@M-^Sderived motor neurons and astrocytes, together with activated microglia as a stress paradigm. Hit compounds were discovered and the transcriptional response on activated astrocytes cells was tested. Neural stem cell (NSC)-derived astrocytes (AC) were activated with LPS and IFN-M-NM-3 and treated with hit compound for 4 hr.

ORGANISM(S): Mus musculus

SUBMITTER: Susanne HM-CM-6ing

PROVIDER: E-GEOD-37611 | biostudies-arrayexpress |

REPOSITORIES: biostudies-arrayexpress

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Discovery of inhibitors of microglial neurotoxicity acting through multiple mechanisms using a stem-cell-based phenotypic assay.

Höing Susanne S Rudhard York Y Reinhardt Peter P Glatza Michael M Stehling Martin M Wu Guangming G Peiker Christiane C Böcker Alexander A Parga Juan A JA Bunk Eva E Schwamborn Jens C JC Slack Mark M Sterneckert Jared J Schöler Hans R HR

Cell stem cell 20121011 5

Stem cells, through their ability to both self-renew and differentiate, can produce a virtually limitless supply of specialized cells that behave comparably to primary cells. We took advantage of this property to develop an assay for small-molecule-based neuroprotection using stem-cell-derived motor neurons and astrocytes, together with activated microglia as a stress paradigm. Here, we report on the discovery of hit compounds from a screen of more than 10,000 small molecules. These compounds ac ...[more]

PMID: 23064101

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