Browse
Submit Data
Databases
API
Help

Unknown,Transcriptomics,Genomics,Proteomics

Dataset Information

43 Views

0 Connections

0 Citations

0 Reanalyses

0 Downloads

Omics score: 0

The effect of alpha, beta, gamma interferon receptors on host reponse to influenza virus in mouse embryonic fibroblasts

ABSTRACT: The objective of this study was to examine the host transcriptional response to high and low pathogenecity viruses in mouse embryonic fibroblasts (MEFs) isolated from different mouse genetic backgrounds to further understand the contribution of interferon signaling pathways to host response to influenza. WT, IFNgR-/-, IFNabR-/-, or IFNabgR-/- MEFs were mock-infected or infected with the A/WSN/33 (WSN), reconstructed 1918 (r1918), or A/Vietnam/1203/2004 (VN1203) strains of influenza virus at an MOI of 2 PFU/cell. At 24 h p.i., total RNA was isolated from the cells and used for microarray hybridization. Experiments with VN1203 and r1918 were performed seperately (experiment a) from experiments with WSN (experiment b). Appropriate mock samples were harvested in each experiment. All gene expression profiles are compared to time-, experiment-, and genotype-matched mock-infected samples.

ORGANISM(S): Mus musculus

SUBMITTER: Richard Green

PROVIDER: E-GEOD-37665 | biostudies-arrayexpress |

REPOSITORIES: biostudies-arrayexpress

ACCESS DATA

Json Xml

Similar Datasets

C646, a Novel p300/CREB-Binding Protein-Specific Inhibitor of Histone Acetyltransferase, Attenuates Influenza A Virus Infection

Project description:Whole-genome data was developed from influenza virus infected A549 cells to better characterize the effect of C646 on influenza virus infection A549 cells were treated with C646 or DMSO for 10 h, and then were infected with A/WSN/33 virus (WSN; H1N1) at a multiplicity of infection (MOI) 2. A549 cells for microarray studies were collected at different times. The gene expression in A549 cells was compared between C646-treated group and DMSO-treated group.

2016-01-07 | E-GEOD-72503 | biostudies-arrayexpress

Host cell gene expression in Influenza A/WSN/33 (H1N1) infected MDCK cells at 2, 4 and 6 hours post infection

Project description:We used the microarray data to analyze host cells response on MDCK cells infected with A/WSN/33 (H1N1) A/WSN/33 (H1N1) infected MDCK cells were harvested at 2, 4, and 6 hpi and RNA extraction was performed using standard protocol as described by Affymetrix. The aim of this experiment is to analyze host response to Influenza A/WSN/33 (H1N1) infection.

2013-01-03 | E-GEOD-31509 | biostudies-arrayexpress

Host cell gene expression in Influenza A/WSN/33 (H1N1) infected MDCK cells at 10 hour post infection

Project description:We used the microarray data to analyze host cells response on MDCK cells infected with A/WSN/33 (H1N1) A/WSN/33 (H1N1) infected MDCK cells were harvested at 10 hpi and RNA extraction was performed using standard protocol as described by Affymetrix. The aim of this experiment is to analyze host response to Influenza A/WSN/33 (H1N1) infection.

2013-01-03 | E-GEOD-31510 | biostudies-arrayexpress

Host cell gene expression in Influenza A/WSN/33 (H1N1) infected A549 cells at 10 hour post infection

Project description:We used the microarray data to analyze host cells response on A549 cells infected with A/WSN/33 (H1N1) The A/WSN/33 (H1N1) infected A549 cells were harvested at 10 hpi and RNA extraction was performed using standard protocol as described by Affymetrix. The aim of this experiment is to analyze host response to Influenza A/WSN/33 (H1N1) infection.

2013-01-03 | E-GEOD-31470 | biostudies-arrayexpress

Host cell gene expression in Influenza A/WSN/33 (H1N1) infected CEF cells at 10 hour post infection

Project description:We used the microarray data to analyze host cells response on CEF cells infected with A/WSN/33 (H1N1) A/WSN/33 (H1N1) infected CEF cells were harvested at 10 hpi and RNA extraction was performed using standard protocol as described by Affymetrix. The aim of this experiment is to analyze host response to Influenza A/WSN/33 (H1N1) infection.

2013-01-03 | E-GEOD-31499 | biostudies-arrayexpress

ICL0011 - Host response in human Calu-3 cells to infection with A/Vietnam/1203-CIP048_RG3/2004 (H5N1) (PB1-F2 deletion), A/Vietnam/1203-CIP048_RG3/2004 (H5N1) (PB2-627E mutant) or WT: A/Vietnam/1203/2004 (H5N1)

Project description:Differential expression was determined in Calu-3 cells between mock infected and infection with one of 3 Influenza viruses (wild-type VN1203, VN1203 mutant PB1-F2del, VN1203 mutant PB2-627E) at different times post infection.

2013-01-05 | GSE43203 | GEO

The response of murine primary airway epithelial cells to Influenza infection and the importance of Interferon type I signaling in this response [mAEC].

Project description:To investigate how murine airway epithelial cells respond to Influenza infection and how important interferon type I signaling is for this response, we harvested airway epithelial cells from the tracheas of wild type, interferon type I knockout(IFNaR-/-) and STAT1 knockout (STAT1-/-) mice and cultured them as previously described (Pickles et al,1998) in polarized airway epithelial cell cultures (mAECs). Triplicate mAECs from each type of mouse (wt,IFNaR-/-,STAT1-/-) were infected with 2X105 PFUs Influenza A (WSN) for 2h or mock inoculated and harvested 24h after infection. Triplicate murine polarized airway epithelial cell cultures from wild type, IFNaR-/- or STAT1-/- mice were mock treated or infected with 2x10^5 PFUs of Influenza A (WSN) for 2h and harvested 24 h post infection.

2012-03-12 | E-GEOD-32137 | biostudies-arrayexpress

Host cell gene expression in Influenza A/WSN/33 (H1N1) infected A549 cells at 2, 4 and 6 hours post infection

Project description:We used the microarray data to analyze host cells response on A549 cells infected with A/WSN/33 (H1N1) The A/WSN/33 (H1N1) infected A549 cells were harvested at 2, 4 and 6 hpi and RNA extraction was performed using standard protocol as described by Affymetrix. The aim of this experiment is to analyze host response to Influenza A/WSN/33 (H1N1) infection.

2013-01-03 | E-GEOD-31469 | biostudies-arrayexpress

Host cell gene expression in Influenza A/WSN/33 (H1N1) infected CEF cells at 2, 4, 6, and 8 hours post infection

Project description:We used the microarray data to analyze host cells response on CEF cells infected with A/WSN/33 (H1N1) A/WSN/33 (H1N1) infected CEF cells were harvested at 2, 4, 6 and 8 hpi and RNA extraction was performed using standard protocol as described by Affymetrix. The aim of this experiment is to analyze host response to Influenza A/WSN/33 (H1N1) infection.

2013-01-03 | E-GEOD-31476 | biostudies-arrayexpress

Lethal dissemination of H5N1 influenza virus is associated with dysregulation of inflammation and lipoxin signaling in a mouse model of infection

Project description:Periodic outbreaks of highly pathogenic avian H5N1 influenza viruses and the current H1N1 pandemic highlight the need for a more detailed understanding of influenza virus pathogenesis. To investigate the host transcriptional response induced by pathogenic influenza viruses, we used a functional-genomics approach to compare gene expression profiles in lungs from wild-type 129S6/SvEv and interferon receptor (IFNR) knockout mice infected with either the fully reconstructed H1N1 1918 pandemic virus (1918) or the highly pathogenic avian H5N1 virus Vietnam/1203/04 (VN/1203). Eight- to 10-week-old female wild-type and IFNR1-/- mice (on a 129S6/SvEv background) were anesthetized by intraperitoneal injection of 0.2 ml of 2,2,2-tribromoethanol in tert-amylalcohol (Avertin; Sigma-Aldrich, Milwaukee, WI). Ten times the 50% lethal dose (LD50), 3.2 × 10^4 PFU (1918) or 7 × 10^3 PFU (VN/1203), in 50 μl of infectious virus diluted in phosphate-buffered saline (PBS) was inoculated intranasally (i.n.). Lung tissue was harvested for microarray analysis from infected animals at 1, 3, and 4 days post-innoculation. For RNA isolation, lungs were frozen in individual tubes and stored in solution D (4 M guanidinium thiocyanate, 25 mM sodium citrate, 0.5% sarcosyl, 0.1 M β-mercaptoethanol). Separate microarrays were run for each infected mouse. This included 2 animals/time point for 1918 virus-infected mice (24 animals total) or 3 animals/time point for VN/1203-infected mice (36 animals total). Lung tissue from three uninfected wild type 129S6/SvEv mice was collected as a mock control. Equal masses of total RNA from the lung tissue of the three mice were pooled prior to being run on microarray. Two-channel microarrays were used to determine gene expression in the lungs. For each individual infected lung, gene expression from an infected lung was compared to gene expression from the pooled RNA from the mock control.

2013-07-16 | E-GEOD-48890 | biostudies-arrayexpress

OmicsDI is part of the ELIXIR infrastructure

OmicsDI is an Elixir interoperability service. Learn more ›

Tweets

OmicsDI Databases

PRIDE
PeptideAtlas
MassIVE
JPOST Repository
Physiome Model Repository

EGA
EVA
ENA
LINCS
PAXDB
Cell Collective

MetaboLights
Metabolomics Workbench
MetabolomeExpress
GNPS
BioModels
FAIRDOMHub

ArrayExpress
dbGaP
ExpressionAtlas
GEO
NODE

Information

Databases
Help
API
Contact us
Code on GitHub
Terms of use
Submit Data