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Gene Expression Effects of IL-13 on Primary Human Airway Epithelial Cells

ABSTRACT: Primary culture airway epithelial cells, grown under physiologic air-liquid interface conditions, with, or without IL-13 in order to study the effects of this cytokine on mucous cell metaplasia, an important feature of asthma and COPD. Keywords: IL13, mucus, goblet cell RNA was isolated from primary culture airway epithelial cells grown at air-liquid interface, treated with or without IL-13 for 21 days.

ORGANISM(S): Homo sapiens

SUBMITTER: Anand Patel

PROVIDER: E-GEOD-37693 | biostudies-arrayexpress |

REPOSITORIES: biostudies-arrayexpress

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IL-13-induced airway mucus production is attenuated by MAPK13 inhibition.

Alevy Yael G YG Patel Anand C AC Romero Arthur G AG Patel Dhara A DA Tucker Jennifer J Roswit William T WT Miller Chantel A CA Heier Richard F RF Byers Derek E DE Brett Tom J TJ Holtzman Michael J MJ

The Journal of clinical investigation 20121126 12

Increased mucus production is a common cause of morbidity and mortality in inflammatory airway diseases, including asthma, chronic obstructive pulmonary disease (COPD), and cystic fibrosis. However, the precise molecular mechanisms for pathogenic mucus production are largely undetermined. Accordingly, there are no specific and effective anti-mucus therapeutics. Here, we define a signaling pathway from chloride channel calcium-activated 1 (CLCA1) to MAPK13 that is responsible for IL-13-driven muc ...[more]

PMID: 23187130

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