Ontology highlight
ABSTRACT:
ORGANISM(S): Homo sapiens
SUBMITTER: Nicholas Ingolia
PROVIDER: E-GEOD-37744 | biostudies-arrayexpress |
REPOSITORIES: biostudies-arrayexpress
Ingolia Nicholas T NT Brar Gloria A GA Rouskin Silvia S McGeachy Anna M AM Weissman Jonathan S JS
Nature protocols 20120726 8
Recent studies highlight the importance of translational control in determining protein abundance, underscoring the value of measuring gene expression at the level of translation. We present a protocol for genome-wide, quantitative analysis of in vivo translation by deep sequencing. This ribosome profiling approach maps the exact positions of ribosomes on transcripts by nuclease footprinting. The nuclease-protected mRNA fragments are converted into a DNA library suitable for deep sequencing usin ...[more]