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Analysis of differential genetic expression by dietary intervention using moderate-carbohydrate restriction diet (MCD) using a transgenic liver cancer model developed by a simple hydrodynamic transfection method.

ABSTRACT: We performed survival analysis of control and MCD groups, and explored underlying tumor suppression mechanisms after dietary intervention, focused on alterations in the energy-dependent signaling pathways, histone modifications, and global gene expression differences on cDNA microarray study. Five- week- old male C57BL6 mice were randomly divided into two groups and fed control diet (control group, LabDiet, Brentwood, MO, USA) or moderate restriced carbohydrate diet formula (MCD, Treat group) in a specific pathogen free zone. All procedures were approved by the institutional animal use and care committee. Following a preliminary feeding of each diet formula for two week, HrasG12V / shp53 / GFP4 gene containing transposon vector were injected into mouse tail vein by hydrodynamic injection method. After 4 weeks of diet supplementation, all mice were sacrificed. Mouse liver tissue was excised for microarray analysis.

ORGANISM(S): Mus musculus

SUBMITTER: Jong-Doo Lee

PROVIDER: E-GEOD-37762 | biostudies-arrayexpress |

REPOSITORIES: biostudies-arrayexpress

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