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Expression data from AK4 overexpression in CL1-0 lung cancer cell line and AK4 knockdown in CL1-5 lung cancer cell line

ABSTRACT: We demonstrate that AK4 down-regulation shRNAs significantly reduced cell migration and invasion in highly invasive lung cancer cell lines in vitro, as well as in lung metastases in vivo We used microarrays to analyze the AK4 regulated gene expression underlying invasion-metastasis cascade. CL1-0 lung adenocarcinoma cells with AK4 overexpression and CL1-5 cells with AK4 knockdown were selected for RNA extraction and hybridization on Affymetrix microarrays.

ORGANISM(S): Homo sapiens

SUBMITTER: Michael Hsiao

PROVIDER: E-GEOD-37903 | biostudies-arrayexpress |

REPOSITORIES: biostudies-arrayexpress

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Adenylate kinase-4 is a marker of poor clinical outcomes that promotes metastasis of lung cancer by downregulating the transcription factor ATF3.

Jan Yi-Hua YH Tsai Hong-Yuan HY Yang Chih-Jen CJ Huang Ming-Shyan MS Yang Yi-Fang YF Lai Tsung-Ching TC Lee Chien-Hsin CH Jeng Yung-Ming YM Huang Chi-Ying CY Su Jen-Liang JL Chuang Yung-Jen YJ Hsiao Michael M

Cancer research 20120920 19

Biomarkers predicting metastatic capacity might assist the development of better therapeutic strategies for aggressive cancers such as lung cancer. In this study, we show that adenylate kinase-4 (AK4) is a progression-associated gene in human lung cancer that promotes metastasis. Analysis of published microarray data showed that AK4 was upregulated in lung adenocarcinoma compared with normal cells. High AK4 expression was associated with advanced stage, disease recurrence and poor prognosis. Los ...[more]

PMID: 23002211

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Expression data from CL1-0 and CL1-5 lung cancer cell line.

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Expression data from FDFT1 overexpression lung cancer cell lines.

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Expression data from CL1-0 and CL1-5 lung cancer cell line.

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Expression data from KSRP overexpression and knockdown lung cancer cell

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Expression data from AK4 overexpression in CL1-0 lung cancer cell line and AK4 knockdown in CL1-5 lung cancer cell line

Project description:We demonstrate that AK4 down-regulation shRNAs significantly reduced cell migration and invasion in highly invasive lung cancer cell lines in vitro, as well as in lung metastases in vivo We used microarrays to analyze the AK4 regulated gene expression underlying invasion-metastasis cascade.

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Expression data from PSAT1 overexpression in CL1-0 lung cancer cell line and PSAT1 knockdown in CL1-5 lung cancer cell line.

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