Unknown,Transcriptomics,Genomics,Proteomics

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MicroRNA binding by wildtype Ago2 and mutant Ago2 in human cells


ABSTRACT: Ago2 binds mature microRNAs (miRNAs) to regulate gene expression. A mutant Ago2 is constructed to render it unable to bind miRNAs and inhibit mRNA translation. Human 293T cells were transiently transfected with a plasmid that encoded a FLAG-tagged wildtype human Ago2 or mutant Ago2. FLAG IP was preformed, and assoicated RNA was isolated and subjected miRNA microarray analysis.

ORGANISM(S): Homo sapiens

SUBMITTER: Yan Zeng 

PROVIDER: E-GEOD-37941 | biostudies-arrayexpress |

REPOSITORIES: biostudies-arrayexpress

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