MicroRNA binding by wildtype Ago2 and mutant Ago2 in human cells
Ontology highlight
ABSTRACT: Ago2 binds mature microRNAs (miRNAs) to regulate gene expression. A mutant Ago2 is constructed to render it unable to bind miRNAs and inhibit mRNA translation. Human 293T cells were transiently transfected with a plasmid that encoded a FLAG-tagged wildtype human Ago2 or mutant Ago2. FLAG IP was preformed, and assoicated RNA was isolated and subjected miRNA microarray analysis.
ORGANISM(S): Homo sapiens
SUBMITTER: Yan Zeng
PROVIDER: E-GEOD-37941 | biostudies-arrayexpress |
REPOSITORIES: biostudies-arrayexpress
ACCESS DATA