Unknown,Transcriptomics,Genomics,Proteomics

Dataset Information

0

Dnmt3a regulates both cell proliferation and differentiation of mouse neural stem cells


ABSTRACT: DNA methylation is known to regulate cell differentiation and neuronal function in vivo. Here we examined whether deficiency of a de novo DNA methyltransferase, Dnmt3a, affects in vitro differentiation of mouse embryonic stem cells (mESCs) to neuronal and glial cell lineages. We found that Dnmt3a-/- neural stem cells (NSCs) derived from mESCs have globally reduced methylcytosine levels and precociously differentiates into astrocytes and oligodendrocytes, consistent with our previous findings in the more severely hypomethylated Dnmt1-/- NSCs. Moreover, Dnmt3a-/- NSC proliferation rate was significantly increased when compared to control. Thus, our work revealed a novel role for Dnmt3a in regulating both timing of neural cell differentiation and cell proliferation in NSCs. Dnmt3a KO vs. WT neural stem cells; 3 biological replicates of each.

ORGANISM(S): Mus musculus

SUBMITTER: Kevin Huang 

PROVIDER: E-GEOD-38035 | biostudies-arrayexpress |

REPOSITORIES: biostudies-arrayexpress

Similar Datasets

2010-06-24 | E-GEOD-22476 | biostudies-arrayexpress
2015-04-01 | E-GEOD-36997 | biostudies-arrayexpress
2010-06-25 | E-GEOD-22475 | biostudies-arrayexpress
2018-01-01 | E-MTAB-5586 | biostudies-arrayexpress
2019-04-11 | E-MTAB-7428 | biostudies-arrayexpress
2014-05-02 | E-GEOD-56988 | biostudies-arrayexpress
2010-07-29 | E-GEOD-22473 | biostudies-arrayexpress
2012-01-30 | E-GEOD-35390 | biostudies-arrayexpress
2013-06-09 | E-GEOD-46791 | biostudies-arrayexpress
2013-06-09 | E-GEOD-46792 | biostudies-arrayexpress