Unknown,Transcriptomics,Genomics,Proteomics

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Transcription profiling of embryonic kidneys from wild type and Hoxa11, Hoxd11 compound null mice throughout development to characterize Hoxa11/Hoxd11 mutant kidney development

ABSTRACT: Complete (whole) embryonic kidneys were dissected from wild type and Hoxa11, Hoxd11 compound null embryos throughout development. Targets from two biological replicates of each were generated and the expression profiles were determined using Affymetrix MOE430A and MOE430B arrays. Comparisons between normal and mutant and comparisons of development samples identified global patterns of gene regulation in kidney development Experiment Overall Design: Embryonic metanephric kidney samples throughout development were analyzed based on normalization to adult kidney samples. In addition, Hoxa11, Hoxd11 compound null embronic kidneys were normalized to wild type embryonic controls. All developmental and adult stages were represented in biological (seperate embryo/animal replicate).

Normalized data files were not included because there appears to be a mismatch between the composite sequence identifiers in some of them and the array design selected.

ORGANISM(S): Mus musculus

SUBMITTER: Kristopher Robert Schwab

PROVIDER: E-GEOD-3808 | biostudies-arrayexpress |

REPOSITORIES: biostudies-arrayexpress

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Publications

Comprehensive microarray analysis of Hoxa11/Hoxd11 mutant kidney development.

Schwab Kristopher K Hartman Heather A HA Liang Hung-Chi HC Aronow Bruce J BJ Patterson Larry T LT Potter S Steven SS

Developmental biology 20060411 2

The Hox11 paralogous genes play critical roles in kidney development. They are expressed in the early metanephric mesenchyme and are required for the induction of ureteric bud formation and its subsequent branching morphogenesis. They are also required for the normal nephrogenesis response of the metanephric mesenchyme to inductive signals from the ureteric bud. In this report, we use microarrays to perform a comprehensive gene expression analysis of the Hoxa11/Hoxd11 mutant kidney phenotype. We ...[more]

PMID: 16581055

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Dataset Information

Transcription profiling of embryonic kidneys from wild type and Hoxa11, Hoxd11 compound null mice throughout development to characterize Hoxa11/Hoxd11 mutant kidney development

Publications

Comprehensive microarray analysis of Hoxa11/Hoxd11 mutant kidney development.

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