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Expression data from Oryza sativa and Arabidopsis thaliana

ABSTRACT: The goal of this work is to identify the gene regulatory hubs that control nitrogen-use in Oryza sativa, one of the most important crop plants, by using a combination of genomics, bioinformatics and systems biology approaches. Here, we evaluate the role of bZIP1, a transcription factor involved in light and nitrogen sensing, by exposing wild-type (WT) and bZIP1 T-DNA null mutant plants to a combinatorial space of N and L treatment conditions. We use ANOVA analysis combined with clustering and Boolean modeling, to evaluate the role of bZIP1 in mediating L and N signaling genome-wide. We also study the interspecies conservation, comparing rice with Arabidopsis thaliana nitrogen transcriptomes, to help identify conserved nitrogen regulation.

ORGANISM(S): Arabidopsis thaliana

SUBMITTER: Mariana Obertello

PROVIDER: E-GEOD-38102 | biostudies-arrayexpress |

REPOSITORIES: biostudies-arrayexpress

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Expression data from Arabidopsis thaliana

Project description:Nitrogen and light are two major regulators of plant metabolism and development. While genes involved in the control of each of these signals have begun to be identified, regulators that integrate gene responses to nitrogen and light signals have yet to be determined. Here, we evaluate the role of bZIP1, a transcription factor involved in light and nitrogen sensing, by exposing wild-type (WT) and bZIP1 T-DNA null mutant plants to a combinatorial space of N and L treatment conditions. We use ANOVA analysis combined with clustering and Boolean modeling, to evaluate the role of bZIP1 in mediating L and N signaling genome-wide. Arabidopsis thaliana were growth on basal MS salts (custom-made; GIBCO) with 0.5 mM KNO3, 3 mM sucrose and 0.8% BactoAgar at pH 5.7. After 14 days under long-day (16 h light: 8 h dark) conditions with light intensity of 50 μE.m-2.s-1 and at 22°C, plants were transferred to new plates containing 20 mM KNO3 and 20 mM NH4NO3 (referred here as 1xN: concentrations in MS media) in the absence or presence of light for 2 h at the start of their light cycle.

2010-08-25 | E-GEOD-21601 | biostudies-arrayexpress

Root nitrate response of wild type plants and afb3-1 mutant plants in Arabidopsis thaliana

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2012-04-30 | E-GEOD-35544 | biostudies-arrayexpress

Root nitrate response of Col-0 plants and tga1/tga4 mutant plants

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2013-03-31 | E-GEOD-43011 | biostudies-arrayexpress

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2022-01-06 | PXD027569 | Pride

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2015-02-28 | E-GEOD-53564 | biostudies-arrayexpress

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Expression analysis of Gordonia sp. KTR9 upon exposure to RDX and Nitrogen limitation

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2013-02-06 | E-GEOD-42340 | biostudies-arrayexpress

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