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Identification of transcripts predominantly expressed in cultured chicken primordial germ cells and embryonic fibroblasts.

ABSTRACT: The present investigation was to identify the signaling and metabolic pathways of expressed genes by microarray comparison between Primordial Germ Cells (PGCs) and their somatic counterpart, chicken embryonic fibroblasts (CEFs). We identified a total of 2,605 expressed transcripts. Among these, 1,197 were predominantly expressed in PGCs, and 1,408 were predominantly expressed in CEFs. Cell culture and microarray data generation were performed in triplicate. Blood PGCs from E2.5 embryos (N=20 for each replication) and CEFs from E6.5 embryos (N=5 for each replication) were cultured in appropriate culture medium. Total RNA was extracted from cultured PGCs (1,500,000 cells for each replication) and cultured CEFs (6,000,000 cells for each replication) with a Qiagen RNeasy kit. About 5 M-BM-5g of total RNA from each replication was used for labeling. Probe synthesis from total RNA samples, hybridization, detection, and scanning were performed according to standard protocols from Affymetrix.

ORGANISM(S): Gallus gallus

SUBMITTER: JAE HAN 

PROVIDER: E-GEOD-38168 | biostudies-arrayexpress |

REPOSITORIES: biostudies-arrayexpress

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Publications

Gene pathways and cell cycle-related genes in cultured avian primordial germ cells.

Rengaraj D D   Lee B R BR   Choi J W JW   Lee S I SI   Seo H W HW   Kim T H TH   Choi H J HJ   Song G G   Han J Y JY  

Poultry science 20121201 12

Primordial germ cells (PGC) from early embryos are applicable to various kinds of research, including the production of transgenic animals. Primordial germ cells eventually migrate and differentiate into germ cells in the gonads, where they settle and rapidly proliferate. However, the proliferation rate of PGC is low in early embryos, and there are many significant pathways that mediate PGC activity. Therefore, in vitro culture of PGC from early embryos with efficient growth factors has been nec  ...[more]

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