Unknown,Transcriptomics,Genomics,Proteomics

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Hog1 controls global reallocation of RNA Pol II upon osmotic shock

ABSTRACT: When challenged with osmotic shock, S. cerevisiae induces hundreds of genes, despite a global reduction in transcriptional capacity. The mechanisms that regulate this rapid reallocation of transcriptional resources are not known. Here we show that redistribution of RNA Pol II upon stress requires the stress-responsive MAP kinase Hog1. We find that Hog1 and RNA Pol II co-localize to open reading frames that bypass global transcriptional repression, and that these targets are specified by two osmotic stress-responsive transcription factors. The combination of reduced global transcription with a gene-specific override mechanism allows cells to rapidly switch their transcriptional program in response to stress. ChIP-sequencing of S. cervisiae RNA Pol II, Hog1, Sko1 and Hot1 Processed data file descriptions: ORFcounts.txt: table of summed ChIP-seq reads that align to each ORF (normalized by reads per kilobase per million) promoter_counts.txt: table of summed ChIP-seq reads that align to each promoter (1kb upstream, normalized by reads per kilobase per million) downstream_counts.txt: table of summed ChIP-seq reads that align 3' regions (50-500 bp downstream, normalized by reads per kilobase per million) Sko1_peak_list.txt: table of peaks found by PeakSeq Hot1_peak_list.txt: table of peaks found by PeakSeq

ORGANISM(S): Saccharomyces cerevisiae

SUBMITTER: Kristen Cook

PROVIDER: E-GEOD-38208 | biostudies-arrayexpress |

REPOSITORIES: biostudies-arrayexpress

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Hog1 controls global reallocation of RNA Pol II upon osmotic shock in Saccharomyces cerevisiae.

Cook Kristen E KE O'Shea Erin K EK

G3 (Bethesda, Md.) 20120901 9

When challenged with osmotic shock, Saccharomyces cerevisiae induces hundreds of genes, despite a concurrent reduction in overall transcriptional capacity. The stress-responsive MAP kinase Hog1 activates expression of specific genes through interactions with chromatin remodeling enzymes, transcription factors, and RNA polymerase II. However, it is not clear whether Hog1 is involved more globally in modulating the cell's transcriptional program during stress, in addition to activating specific ge ...[more]

PMID: 22973550

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Dataset Information

Hog1 controls global reallocation of RNA Pol II upon osmotic shock

Publications

Hog1 controls global reallocation of RNA Pol II upon osmotic shock in Saccharomyces cerevisiae.

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