Unknown,Transcriptomics,Genomics,Proteomics

Dataset Information

0

Identification of maternally-loaded RNA transcripts in unfertilized eggs of Tribolium castaneum

ABSTRACT: Using a tiled whole-genome microarray, we found that 58.2% of Tribolium castaneum genes are maternally loaded into eggs. Comparison of known Drosophila melanogaster maternal genes to our results showed widespread conservation of maternal function with T. castaneum. We also found many T. castaneum genes with previously identified gender or tissue specific expression were also maternally loaded into eggs. The microarray design also allowed the detection of 2315 and 4060 novel transcriptionally active regions greater in length than 100 bp in unfertilized and fertilized T. castaneum eggs, respectively. The primary objective of this study was to identify expressed regions of the Tribolium castaneum genome in unfertilized and fertilized eggs using a whole-genome tiled microarray. The whole RNA of 3 samples of virgin laid eggs and 3 samples of fertilized eggs were compaired.

ORGANISM(S): Tribolium castaneum

SUBMITTER: Jacqueline Lopez 

PROVIDER: E-GEOD-38245 | biostudies-arrayexpress |

REPOSITORIES: biostudies-arrayexpress

Json Xml
altmetric image

Publications

Identification of maternally-loaded RNA transcripts in unfertilized eggs of Tribolium castaneum.

Preuss Kevin M KM   Lopez Jacqueline A JA   Colbourne John K JK   Wade Michael J MJ  

BMC genomics 20121127

<h4>Background</h4>Maternal RNAs play a critical role in early development. Variation in the diversity and levels of maternally derived gene transcripts may be central to the origin of phenotypic novelty -- a longstanding problem in evolution and development. By studying maternal transcriptomes within and between divergent species, a better understanding of the evolutionary forces acting on maternal RNA allocation is possible.<h4>Results</h4>We present the first maternal transcriptome of the red  ...[more]

Similar Datasets

Transcriptomics Identification of maternally-loaded RNA transcripts in unfertilized eggs of Tribolium castaneum
2013-05-06 | GSE38245 | GEO
Genomics Tribolium castaneum
| PRJNA167563 | ENA
Transcriptomics Broodstock - transcription profiling of maternal RNA in Atlantic cod eggs and embryos after female broodstock stress
2012-11-01 | E-MTAB-1296 | biostudies-arrayexpress
Transcriptomics Deep sequencing of small RNAs from Tribolium castaneum
2010-12-29 | E-GEOD-26036 | biostudies-arrayexpress
Genomics Drosophila melanogaster
| PRJNA104813 | ENA
Genomics Deep sequencing of small RNAs from Tribolium castaneum
2010-12-29 | GSE26036 | GEO
Transcriptomics Genome-wide survey of transcriptional differences between males and females of Tribolium castaneum, the red flour beetle
2010-05-27 | GSE18087 | GEO
Transcriptomics The extraembryonic serosa is a frontier epithelium providing the insect egg with a full-range innate immune response
2014-12-09 | GSE54018 | GEO
Genomics Mus musculus
| PRJNA91295 | ENA
Proteomics Egg MVBs elicit an LC3-associated phagocytosis-like pathway to degrade paternal mitochondria after fertilization
2023-11-06 | MSV000093306 | MassIVE