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MRNA expression in iPS cells generated by a synthetic self-replicative RNA

ABSTRACT: We generated iPS cells with a synthetic self-replicative RNA that expresses four independent reprogramming factors (OCT4, KLF4, SOX2 and either c-MYC or GLIS1). We performed whole genome RNA sequencing (RNA-seq) of iPS cell clones, parental BJ and HUES9 ES cell controls. All iPS cell clones analyzed by RNA-seq showed unsupervised hierarchical clustering and expression signatures characteristic of human HUES9 ES cells that were highly divergent from parental human fibroblasts. RNA-seq in two OKS-iM iPS clones (generated from OCT4, KLF4, SOX2 and cMYC expressing RNA replicon), two OKS-iG clones (generated from OCT4, KLF4, SOX2 and GLIS1 expressing RNA replicon), HUES9 and BJ cells.

ORGANISM(S): Homo sapiens

SUBMITTER: Hai-Ri Li

PROVIDER: E-GEOD-38265 | biostudies-arrayexpress |

REPOSITORIES: biostudies-arrayexpress

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Efficient generation of human iPSCs by a synthetic self-replicative RNA.

Yoshioka Naohisa N Gros Edwige E Li Hai-Ri HR Kumar Shantanu S Deacon Dekker C DC Maron Cornelia C Muotri Alysson R AR Chi Neil C NC Fu Xiang-Dong XD Yu Benjamin D BD Yu Benjamin D BD Dowdy Steven F SF

Cell stem cell 20130801 2

The generation of human induced pluripotent stem cells (iPSCs) holds great promise for the development of regenerative medicine therapies to treat a wide range of human diseases. However, the generation of iPSCs in the absence of integrative DNA vectors remains problematic. Here, we report a simple, highly reproducible RNA-based iPSC generation approach that utilizes a single, synthetic self-replicating VEE-RF RNA replicon that expresses four reprogramming factors (OCT4, KLF4, and SOX2, with c-M ...[more]

PMID: 23910086

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