Unknown,Transcriptomics,Genomics,Proteomics

Dataset Information

0

RNA polymerase II collision interrupts convergent transcription (ChIP-seq)


ABSTRACT: Anti-sense non-coding transcripts, genes-within-genes, and convergent gene pairs are prevalent among eukaryotes. The existence of such transcription units raises the question of what happens when RNA polymerase II (RNAPII) molecules collide head-to-head. Here we use a combination of biochemical and genetic approaches in yeast to show that polymerases transcribing opposite DNA strands cannot bypass each other. RNAPII stops, but does not dissociate upon head-to-head collision in vitro, suggesting that opposing polymerases represent insurmountable obstacles for each other. Head-to-head collision in vivo results in RNAPII stopping as well, and removal of collided RNAPII from the DNA template can be achieved via ubiquitylation-directed proteolysis. Indeed, in cells lacking efficient RNAPII poly-ubiquitylation, the half-life of collided polymerases increases, so that these can be detected between convergent genes by ChIP-Seq. These results provide new insight into fundamental mechanisms of gene traffic control, and point to an unexplored effect of anti-sense transcription on gene regulation via polymerase collision. ChIP-Seq of RNA polymerase II was performed with WT and Elongain C deletion mutant (elc1M-bM-^HM-^F) cells. 4H8 antibody against the Rpb1 C-terminal domain was used for RNA polymerase II immunoprecipitation, whilst mouse IgG antibody was used for control immunoprecipitations. Two biological replicates were performed for both WT and elc1M-bM-^HM-^F.

ORGANISM(S): Saccharomyces cerevisiae

SUBMITTER: Wu Wei 

PROVIDER: E-GEOD-38382 | biostudies-arrayexpress |

REPOSITORIES: biostudies-arrayexpress

Similar Datasets

2012-11-30 | E-GEOD-38383 | biostudies-arrayexpress
2012-11-30 | GSE38383 | GEO
2012-11-30 | GSE38382 | GEO
2024-11-05 | GSE219075 | GEO
2024-11-05 | GSE219074 | GEO
2024-11-05 | GSE219073 | GEO
| PRJNA167758 | ENA
| PRJNA167771 | ENA
| PRJNA167772 | ENA
2019-03-29 | PXD012606 | Pride