Unknown,Transcriptomics,Genomics,Proteomics

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Expression data from Andromonoecious poplar flower

ABSTRACT: Exceptionally andromonoecious individuals that flowers appeared to form that structure resembling a carpel attached to the reverse side of stamen cup were detected in strictly dioecious poplar. Andromonoecious poplarswere used to analyze transcript levels accurately. We used microarrays to detail the global programme of gene expression during flower development and identified distinct classes of different-regulated genes between sexes during this process.

ORGANISM(S): Populus tomentosa

SUBMITTER: Yuepeng Song

PROVIDER: E-GEOD-38432 | biostudies-arrayexpress |

REPOSITORIES: biostudies-arrayexpress

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Project description:Winter survival and maintenance strategy is crucial in temperate woody plants. Here, we demonstrate novel aspects of the transcriptional regulations adopted by perennial tree species in winter/dormancy, employing a biochemical and whole transcriptome analysis. As expected, genes related to cold hardiness and defense are over-represented. Interestingly, carbohydrate biosynthesis and transport-related genes were very actively expressed in winter/dormancy. Further biochemical analyses verified the dormancy/winter transcription phenotype. Furthermore, dormancy/winter preferential expression of genes involved in the cell wall biosynthesis/modification, circadian rhythm, the indirect transcriptional regulation (RNA metabolism), and chromatin modification/remodeling were identified. Taken together, regulation of gene expression in the winter survival and maintenance may include not only controlled by promoter binding transcription factors but may also be regulated at the post-transcriptional and chromatin levels. In the first step towards the understanding of molecular mechanisms underlying the winter survival and maintenance of perennial trees, we examined the characteristics of transcription phenotype of the dormancy/winter stem compared to the active growth/summer stem by using a whole transcriptome analysis (GeneChip Poplar Genome Array; Affymetrix). Poplar Genome Array has a total of 61,251 probe sets, representing 57,423 poplar gene models, and allows us to interrogate a total of 41,558 unique gene models because of the probe set redundancy. Slight redundancy of the probe sets within a single chip gave us a unique opportunity to have internal comparisons of the particular genes. Very high reproducibility (R2 > 0.97) between the replicated samples was found. Further confirmation of the GeneChip data was made by semi-quantitative RT-PCR analysis using several genes showing the summer or winter biased expression. Based on these results, further analysis of winter stem transcriptome against summer stem was performed.

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Expression data from Andromonoecious poplar flower

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