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Effect of glyoxylate pathway deficiency on transcriptome profile of Acetobacter aceti

ABSTRACT: Trascriptome profiles in the cells of A. aceti wild-type strain and its isogenic aceA glcB mutant during growth in medium contaning ethanol and glucose was determined by NimbleGen Eukaryotic Expression array (4x72K). Acetobacter aceti NBRC14818 and its isogenic aceA glcB mutant were cultivated in medium containing ethanol and glucose in Erlenmeyer flask with rotary shaking. Total RNA was extracted when optical density at 600 nm was 0.3. The experiment was performed in duplicate independent cultures.

ORGANISM(S): Acetobacter aceti NBRC 14818

SUBMITTER: Hiroyuki Arai

PROVIDER: E-GEOD-38524 | biostudies-arrayexpress |

REPOSITORIES: biostudies-arrayexpress

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Role of the glyoxylate pathway in acetic acid production by Acetobacter aceti.

Sakurai Kenta K Yamazaki Shoko S Ishii Masaharu M Igarashi Yasuo Y Arai Hiroyuki H

Journal of bioscience and bioengineering 20120816 1

Wild-type Acetobacter aceti NBRC 14818 possesses genes encoding isocitrate lyase (aceA) and malate synthase (glcB), which constitute the glyoxylate pathway. In contrast, several acetic acid bacteria that are utilized for vinegar production lack these genes. Here, an aceA-glcB knockout mutant of NBRC 14818 was constructed and used for investigating the role of the glyoxylate pathway in acetate productivity. In medium containing ethanol as a carbon source, the mutant grew normally during ethanol o ...[more]

PMID: 22902276

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