Ontology highlight
ABSTRACT:
ORGANISM(S): Homo sapiens
SUBMITTER: Ge Lin
PROVIDER: E-GEOD-38662 | biostudies-arrayexpress |
REPOSITORIES: biostudies-arrayexpress
Xie Pingyuan P Sun Yi Y Ouyang Qi Q Hu Liang L Tan Yueqiu Y Zhou Xiaoying X Xiong Bo B Zhang Qianjun Q Yuan Ding D Pan Yi Y Liu Tiancheng T Liang Ping P Lu Guangxiu G Lin Ge G
Stem cells (Dayton, Ohio) 20140201 2
Genetic and epigenetic alterations are observed in long-term culture (>30 passages) of human embryonic stem cells (hESCs); however, little information is available in early cultures. Through a large-scale gene expression analysis between initial-passage hESCs (ihESCs, <10 passages) and early-passage hESCs (ehESCs, 20-30 passages) of 12 hESC lines, we found that the DLK1-DIO3 gene cluster was normally expressed and showed normal methylation pattern in ihESC, but was frequently silenced after 20 p ...[more]