Unknown,Transcriptomics,Genomics,Proteomics

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Expression data from human embryonic stem cells(hESCs)


ABSTRACT: To detect the effect of early culture on hESCs, almost all initial-passaged hESCs have normal expression of DLK1-DIO3 gene cluster, the low-passaged hESCs lost the expression of DLK1-DIO3 gene cluster. hESCs were extracted for RNA with TriZOL and hybridization on Affymetrix arrays

ORGANISM(S): Homo sapiens

SUBMITTER: Ge Lin 

PROVIDER: E-GEOD-38662 | biostudies-arrayexpress |

REPOSITORIES: biostudies-arrayexpress

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Publications

Physiological oxygen prevents frequent silencing of the DLK1-DIO3 cluster during human embryonic stem cells culture.

Xie Pingyuan P   Sun Yi Y   Ouyang Qi Q   Hu Liang L   Tan Yueqiu Y   Zhou Xiaoying X   Xiong Bo B   Zhang Qianjun Q   Yuan Ding D   Pan Yi Y   Liu Tiancheng T   Liang Ping P   Lu Guangxiu G   Lin Ge G  

Stem cells (Dayton, Ohio) 20140201 2


Genetic and epigenetic alterations are observed in long-term culture (>30 passages) of human embryonic stem cells (hESCs); however, little information is available in early cultures. Through a large-scale gene expression analysis between initial-passage hESCs (ihESCs, <10 passages) and early-passage hESCs (ehESCs, 20-30 passages) of 12 hESC lines, we found that the DLK1-DIO3 gene cluster was normally expressed and showed normal methylation pattern in ihESC, but was frequently silenced after 20 p  ...[more]

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