Browse
Submit Data
Databases
API
Help

Unknown,Transcriptomics,Genomics,Proteomics

Dataset Information

0 Views

0 Connections

0 Citations

0 Reanalyses

0 Downloads

Omics score: 0

Transcription profiling of human colon Caco-2 cells treated with hydroxytyrosol (HTy) and hydroxytyrosyl ethyl ether (HTy-Et)

ABSTRACT: The anticarcinogenic activity of hydroxytyrosyl ethyl ether (HTy-Et) compared to its precursor hydroxytyrosol (HTy) has been studied in human Caco-2 colon adenocarcinoma cells. Changes in global gene expression in Caco-2 cells exposed to HTy and HTy-Et for 24 h were evaluated using Affymetrix array. Microarray analysis showed that after exposure to HTy and HTy-Et, 451 and 977 genes respectively were differentially expressed compared with untreated cells (P < 0.005; FDR=0). Results show that HTy and its lipophilic ether derivative alter genes related with cancer prevention, which includes inducing cell cycle arrest, promoting apoptosis and enhancing xenobiotic metabolism. Caco-2 cells were treated with 10 M-NM-

ORGANISM(S): Homo sapiens

SUBMITTER: Gema Pereira-Caro

PROVIDER: E-GEOD-38833 | biostudies-arrayexpress |

REPOSITORIES: biostudies-arrayexpress

ACCESS DATA

Json Xml

Similar Datasets

Transcription profiling of human colon Caco-2 cells treated with sulforaphane (SF)

Project description:Effect of sulforaphane (SF) on human colon caco-2 cells after 24h treatment

2005-07-01 | E-MEXP-170 | biostudies-arrayexpress

Transcription profiling of human colon Caco-2 cells treated with hydroxytyrosol (HTy) and hydroxytyrosyl ethyl ether (HTy-Et)

Project description:The anticarcinogenic activity of hydroxytyrosyl ethyl ether (HTy-Et) compared to its precursor hydroxytyrosol (HTy) has been studied in human Caco-2 colon adenocarcinoma cells. Changes in global gene expression in Caco-2 cells exposed to HTy and HTy-Et for 24 h were evaluated using Affymetrix array. Microarray analysis showed that after exposure to HTy and HTy-Et, 451 and 977 genes respectively were differentially expressed compared with untreated cells (P < 0.005; FDR=0). Results show that HTy and its lipophilic ether derivative alter genes related with cancer prevention, which includes inducing cell cycle arrest, promoting apoptosis and enhancing xenobiotic metabolism.

2012-06-20 | GSE38833 | GEO

Transcription profiling by array of human Caco-2 cells with ATP8B1 knock down

Project description:To gain insights whether and if activation of the nuclear receptor FXR is involved in the disease ethiology of ATP8B1 deficiency, we contstructed a Caco-2 ATP8B1 knock down cell line and a Caco-2 empty vector control cell line. Furthermore we expect to gain new insights from transcriptional profiling of ATP8B1 deficient cells.

2010-12-31 | E-TABM-989 | biostudies-arrayexpress

Caco-2 cells cultured in the presence of Lactobacillus fermentum strains AGR1485 or AGR1487

Project description:Transcriptional profiling of Caco-2 cells co-cultured with L. fermentum AGR1487 (isolated from IBD patient), Caco-2 cells co-cultured with L. fermentum AGR1485 (isolated from healthy volunteer), or Caco-2 cells alone (Control). Three-condition experiment with dye swap, A vs B, A vs C, B vs C, 8 biological replicates: A = Control, B = AGR1487, C = AGR1485

2013-05-01 | E-GEOD-46352 | biostudies-arrayexpress

Caco-2 cell gene expression following co-culture with Lactobacillus casei and Bifidobacterium breve

Project description:To characterize how symbiotic bacteria affect the lolecular and cellular mechanisms of epithelial homeostasis, human colonic Caco-2 cells were co-culture with Lactobacillus casei and Bifidobacterium breve. 3 biological replicates were hybridized on Affymetrix HG-U133A

2013-04-09 | E-GEOD-37369 | biostudies-arrayexpress

Expression data from Caco-2 cells expressing TLR4 and associated mutants

Project description:Common missense mutations (D299G, T399I) have been recently identified in the human TLR4 gene. The aim of this study was to determine how TLR4 and associated mutants affect gene expression in Caco-2 cells. We used microarrays to asses gene expression profiles in Caco-2 stably overexpressing TLR4-WT, TLR4-D299G, TLR4-T399I or untransfected. Caco-2 clones stably overexpressing HA-tagged wildtype TLR4-WT, mutant TLR4-D299G or TLR4-T399I were generated. Prior to analysis, cell clones were cultured for 8 days in all experiments. RNA (triplicate) was extracted and hybridized on Affymetrix microarrays.

2011-10-10 | E-GEOD-26226 | biostudies-arrayexpress

Transcription profiling of human intestinal epithelial cell line Caco-2 treated with Lactobacillus plantarum MB452

Project description:Effect of Lactobacillus plantarum MB452 on the gene expression of the intestinal epithelial cell line Caco-2 using a reference design Experiment Overall Design: Effect of Lactobacillus plantarum MB452 on the gene expression of the intestinal epithelial cell line Caco-2 using a reference design

2009-10-09 | E-GEOD-18372 | biostudies-arrayexpress

Modification of miRNA profile in Caco-2 cells by dietary lipids

Project description:The aim of this study was to detect miRNAs modified by biactive dietary lipids in a cellular model of enterocytes. The selected model was Caco-2 cells Differentiated Caco-2 cells were incubated with micelles containing the lipids for 24 and 48 hours. The expression leveles of the miRNAs were compared with cells treated with empty micelles. Four control and choelsterol samples, three DHA samples and two CLA samples were analyzed variables: Control (Empty micelles), Cholesterol 250 M-BM-5M 48h, DHA 200 M-BM-5M 24h, CLA 200M-BM-5M 24h Biological replicates Control: Samples 137, 159, 160, 210 Cholesterol: Samples 150, 151, 152, 219 DHA: Samples 141, 142, 143 CLA: Samples 144, 146

2015-03-01 | E-GEOD-59153 | biostudies-arrayexpress

RNA-seq of human uninfected Caco-2 cells treated with a nutraceutical formula (Solution-3), or vehicle-(saline solution)- control

Project description:Caco-2 cells were plated (1000000 cells) and treated with a nutraceutical formula (Solution-3) at 0.01x concentration or with vehicle (saline solution) as the negative control for the treatment. After 24 hours, the cells were lysed, and their RNA was extracted for RNAseq.

2024-06-20 | E-MTAB-14178 | biostudies-arrayexpress

miRNA expression screening in Liver X Receptor (NR1H3) agonist (GW3965) -treated differentiated Caco-2/TC7 cells

Project description:Differentiated human enterocytic Caco-2/TC7 cells grown on transwell were incubated with LXR agonist GW3965 (5µM) or Vehicle DMSO (1:2000 ; vol:vol) for 48 hours. Total RNA were extracted using mirVana miRNA Isolation Kit (life technologies) then processed with Flash Tag Biotin HSR kit (Affymetrix) and hybridized on an Affymetrix GeneChip miRNA 4.0 Array.

2016-06-03 | E-MTAB-3675 | biostudies-arrayexpress

OmicsDI is part of the ELIXIR infrastructure

OmicsDI is an Elixir interoperability service. Learn more ›

Tweets

OmicsDI Databases

PRIDE
PeptideAtlas
MassIVE
JPOST Repository
Physiome Model Repository

EGA
EVA
ENA
LINCS
PAXDB
Cell Collective

MetaboLights
Metabolomics Workbench
MetabolomeExpress
GNPS
BioModels
FAIRDOMHub

ArrayExpress
dbGaP
ExpressionAtlas
GEO
NODE

Information

Databases
Help
API
Contact us
Code on GitHub
Terms of use
Submit Data