Expression data of gastric cancer by conventional microarray and by suppressive subtractive hybridization coupled to microarray

ABSTRACT: Transcriptional profiling of gastric tumors compared to paired adjacent non-tumoral gastric tissue by two strategies. The first strategy is a conventional microarray assay, while the second strategy involves a previous Suppression Subtractive Hybridization (SSH) followed by a conventional microarray assay. We applied subtractive hybridization followed by microarray analysis to identify tumor-specific low-abundance transcripts. The goal was to determine differences in gene expression profile in tumor samples by both strategies. Two-condition experiment: tumoral vs. adjacent non-tumoral tissues. Biological replicates: 12 tumoral replicates, 12 non-tumoral replicates. The same replicates were used for both strategies (conventional, SSH). Human Universal Reference RNA (Clontech) was used as the reference.

ORGANISM(S): Homo sapiens

SUBMITTER: German Gonzalez 

PROVIDER: E-GEOD-38932 | biostudies-arrayexpress |

REPOSITORIES: biostudies-arrayexpress