Unknown,Transcriptomics,Genomics,Proteomics

Dataset Information

0

Transcriptional analysis of wild-type and delta_dasR mutant of Streptomyces coelicolor grown in soil amended with chitin


ABSTRACT: Genome-wide microarray analysis was performed using RNA extracted from soil cultures of Streptomyces coelicolor A3(2) in the presence or absence of chitin. The vast majority of genes in chitin and amino sugar metabolism, as well as many other genes for carbon and energy, nitrogen and sulfur metabolism, were differentially expressed in response to addition of chitin. Moreover, the gene expressions of eight gene clusters for secondary metabolites were also significantly up-regulated in the chitin amended soil. To reveal the role of a pleiotropic transcriptional regulator, DasR, which has been reported to be involved in regulation of chitin metabolism, antibiotic production and morphological differentiation, the gene expression patterns of wild type and dasR mutant in soil amended with chitin were compared by microarray analysis. The dasR mutation resulted in up-regulation of four antibiotic gene clusters and down-regulation of chitin metabolism. A study using total RNA extracted from soil cultures of Streptomyces ceolicolor A3(2). A whole genome microarray of S. coelicolor (NimbleGen Custom Prokaryotic Gene Expression 72K 4-plex Arrays) was designed and manufactured by Roche (Roche NimbleGen, Madison, WI). Each array contained four sets of 8 sequence-specific 60-mer probes per gene corresponding to 7825 genes from the S. coelicolor A3(2) genome.

ORGANISM(S): Streptomyces coelicolor A3(2)

SUBMITTER: Behnam Nazari 

PROVIDER: E-GEOD-39164 | biostudies-arrayexpress |

REPOSITORIES: biostudies-arrayexpress

altmetric image

Publications

Chitin-induced gene expression in secondary metabolic pathways of Streptomyces coelicolor A3(2) grown in soil.

Nazari Behnam B   Kobayashi Michihiko M   Saito Akihiro A   Hassaninasab Azam A   Miyashita Kiyotaka K   Fujii Takeshi T  

Applied and environmental microbiology 20121102 2


Microarray analyses revealed that the expression of genes for secondary metabolism together with that of primary metabolic genes was induced by chitin in autoclaved soil cultures of Streptomyces coelicolor A3(2). The data also indicated that DasR was involved in the regulation of gene expression for chitin catabolism, secondary metabolism, and stress responses. ...[more]

Similar Datasets

2012-11-07 | GSE39164 | GEO
2011-10-05 | E-GEOD-32642 | biostudies-arrayexpress
2013-12-31 | E-GEOD-31748 | biostudies-arrayexpress
2005-07-26 | GSE2994 | GEO
2010-06-24 | E-GEOD-16800 | biostudies-arrayexpress
2005-07-21 | E-SMDB-2740 | biostudies-arrayexpress
2007-07-12 | E-GEOD-2538 | biostudies-arrayexpress
2008-06-15 | E-GEOD-6990 | biostudies-arrayexpress
2022-03-23 | MSV000089111 | MassIVE
2010-06-24 | E-GEOD-18711 | biostudies-arrayexpress